Na+:K+:ATPase mRNA expression in the kidney during adaptation to sodium intake and furosemide treatment

被引:6
作者
Merino, A
Moreno, G
Mercado, A
Bobadilla, NA
Gamba, G
机构
[1] Univ Nacl Autonoma Mexico, Inst Nacl Ciencia Med & Nutr Salvador Zubiran, Unidad Fisiol Mol, Mexico City 14000, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 14000, DF, Mexico
[3] Inst Nacl Cardiol Ignacio Chavez, Dept Nefrol, Mexico City, DF, Mexico
关键词
Na+: K+: ATPase; mRNA expression; kidney; furosemide; sodium diet;
D O I
10.1016/S0188-4409(00)00102-8
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background. Nephron tubular epithelium possesses the capacity of adaptation to any salt ingestion condition. The mechanism of adaptation is due in part to an increase in the activity of Na+:K+:ATPase at the basolateral membrane. The goal. of the present study was to analyze the long-term regulation of the Na+:K+:ATPase alpha (1)-subunit mRNA expression during changes in NaCl metabolism. Methods. Male Wistar rats given a normal, high, or low NaCl diet,and intraperitoneal administration of the loop diuretic furosemide from 12 h to 7 days were studied. Rats were kept in metabolic cages 4 days before and throughout the study to determine daily urinary electrolyte excretion and osmolarity. At the end of each experimental period, creatinine clearance and serum electrolytes were also measured. Total RNA was extracted from each individual cortex or outer medulla and from pooled inner medullas using the guanidine/cesium chloride method. Na+:K+:ATPase alpha (1)-subunit mRNA expression was assessed by nonradioactive dot-blot analysis. Results. Experimental maneuvers were well tolerated and all groups developed the appropriate renal response to each experimental condition. Urinary sodium excretion was significantly higher in rats administered a high sodium diet or furosemide and lower in rats treated with a low sodium diet after 7 days of treatment. Glomerular filtration rate was similar among all groups. However, the level of expression of the Na+:K+:ATPase alpha (1)-subunit did not change in any model. Conclusions. Nephron adaptation to the modification in NaCl intake or furosemide administration over 7 days did not include changes in Na+:K+:ATPase alpha (1)-subunit mRNA levels. (C) 2001 IMSS. Published by Elsevier Science Inc.
引用
收藏
页码:486 / 492
页数:7
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