Chemiluminescence of lucigenin-tetracycline and its application for sensitive determination of procyanidin

被引:7
作者
Quan, Shuai [1 ,2 ]
Ji, Kaixiang [1 ,2 ]
Liu, Fangshuo [2 ,3 ]
Barkae, Tesfaye Hailemariam [2 ,3 ]
Halawa, Mohamed Ibrahim [4 ,5 ]
Hanif, Saima [6 ]
Lou, Baohua [2 ,3 ]
Li, Jianping [1 ]
Xu, Guobao [1 ,2 ,3 ]
机构
[1] Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[3] Univ Sci & Technol China, Hefei 230026, Peoples R China
[4] Mansoura Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Mansoura 35516, Egypt
[5] Guangdong Lab Artificial Intelligence & Digital E, Shenzhen 518060, Peoples R China
[6] Natl Univ Med Sci NUMS, Dept Biol Sci, Abid Majeed Rd, Rawalpindi 46000, Punjab, India
基金
中国国家自然科学基金;
关键词
Chemiluminescence; Lucigenin; Procyanidin; Tetracycline; CHEMILUMINOMETRIC DETERMINATION; LIQUID-CHROMATOGRAPHY; ANTIBIOTICS; OXIDATION;
D O I
10.38212/2224-6614.3411
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
引用
收藏
页码:293 / 302
页数:11
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