PHASE PLATES FOR TRANSMISSION ELECTRON MICROSCOPY

被引：43

作者：

Danev, Radostin ^{[1
]}

Nagayama, Kuniaki ^{[1
]}

机构：

[1] Natl Inst Nat Sci, Okazaki Inst Integrat Biosci, Okazaki, Aichi 4448787, Japan

来源：

METHODS IN ENZYMOLOGY, VOL 481: CRYO-EM, PART A - SAMPLE PREPARATION AND DATA COLLECTION | 2010年 / 481卷

关键词：

SINGLE-PARTICLE ANALYSIS; COMPLEX OBSERVATION; CONTRAST; RECONSTRUCTION; WAVE;

D O I：

10.1016/S0076-6879(10)81014-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phase plates are a new technique in the field of cryo-electron microscopy. They provide improved contrast and signal-to-noise ratio in images of radiation sensitive specimens. Thin film phase plates are being tested in biological applications and have demonstrated benefits for single particle analysis and cryo-tomography. There are still unsolved problems, such as reliability of manufacturing and deterioration of performance with time. Several other types of phase plates are currently under development and may become available for cryo-microscopy in near future. Presented is a short overview of the current state of the field as well as ideas for the future directions. Also included is a detailed description of the instrumentation requirements and the experimental procedures for phase plate application.

引用

页码：343 / 369

页数：27

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