Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies

被引：22

作者：

Leidenberger, S. ^{[1
]}

Schroeder, Ch. ^{[1
]}

Zani, L. ^{[1
]}

Auste, A. ^{[1
]}

Pinette, M. ^{[2
]}

Ambagala, A. ^{[2
]}

Nikolin, V. ^{[3
]}

de Smit, H. ^{[3
]}

Beer, M. ^{[1
]}

Blome, S. ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Inst Diagnost Virol, Suedufer 10, D-17493 Greifswald, Insel Riems, Germany

[2] Natl Ctr Foreign Anim Dis, Canadian Food Inspect Agcy, Winnipeg, MB R3E 3M4, Canada

[3] Boehringer Ingelheim Vet Res Ctr, D-30559 Hannover, Germany

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

PORCINE-EPIDEMIC-DIARRHEA; TRANSMISSIBLE GASTROENTERITIS; UNITED-STATES; CELL-CULTURES; VIRUS; SWINE; INFECTION; OUTBREAK; CORONAVIRUS; EMERGENCE;

D O I：

10.1038/s41598-017-11160-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect of maternally derived antibodies (MDA), four trial groups were randomly assigned, each consisting of two pregnant sows and their litter. To induce MDA in a subset of piglets, two sows received a cell culture-adapted PEDV strain, and another two sows were inoculated with field material from German PED outbreaks. Four sows stayed naive. Subsequently, all piglets were inoculated with the corresponding PEDV strains at an age of 3 to 6 days, and virus shedding, clinical signs and occurrence of specific antibodies were assessed. Piglets without MDA showed a morbidity of 100% and low lethality, while almost all MDA-positive piglets stayed clinically healthy and showed considerably lower virus shedding. Taken together, the Central European PEDV strains showed rather low virulence under experimental conditions, and pre-inoculation of sows led to a solid protection of their offspring. The latter is the prerequisite for a sow vaccination concept that could help to prevent PED induced losses in the piglet sector.

引用

页数：11

共 52 条

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