Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes

被引：21

作者：

Austwick, Martin R. ^{[1
]}

Clark, Benjamin ^{[1
]}

Mosse, Charles A. ^{[1
]}

Johnson, Kristie ^{[1
]}

Chicken, D. Wayne ^{[1
,2
]}

Somasundaram, Santosh K. ^{[1
,2
]}

Calabro, Katherine W. ^{[3
,4
]}

Zhu, Ying ^{[1
,5
]}

Falzon, Mary ^{[6
]}

Kocjan, Gabrijela ^{[6
]}

Fearn, Tom ^{[5
]}

Bown, Stephen G. ^{[1
]}

Bigio, Irving J. ^{[3
,4
]}

Keshtgar, Mohammed R. S. ^{[7
]}

机构：

[1] UCL, Natl Med Laser Ctr, London W1W 7EJ, England

[2] UCL, Div Surg & Intervent Sci, London W1W 7EJ, England

[3] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[4] Boston Univ, Dept Elect Engn, Boston, MA 02215 USA

[5] UCL, Dept Stat Sci, London W1W 7EJ, England

[6] UCL, Dept Histol, London W1W 7EJ, England

[7] Royal Free Hosp, Sch Med, London, England

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 04期

基金：

美国国家卫生研究院;

关键词：

elastic scattering spectroscopy; backscattering; breast cancer; sentinel lymph node biopsy; IMPRINT CYTOLOGY; BIOPSY; REFLECTANCE; VALIDATION; DYSPLASIA;

D O I：

10.1117/1.3463005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for rapidly detecting metastatic breast cancer within excised sentinel lymph node(s) of the axilla is presented. Elastic scattering spectroscopy (ESS) is a point-contact technique that collects broadband optical spectra sensitive to absorption and scattering within the tissue. A statistical discrimination algorithm was generated from a training set of nearly 3000 clinical spectra and used to test clinical spectra collected from an independent set of nodes. Freshly excised nodes were bivalved and mounted under a fiber-optic plate. Stepper motors raster-scanned a fiber-optic probe over the plate to interrogate the node's cut surface, creating a 20 x 20 grid of spectra. These spectra were analyzed to create a map of cancer risk across the node surface. Rules were developed to convert these maps to a prediction for the presence of cancer in the node. Using these analyses, a leave-one-out cross-validation to optimize discrimination parameters on 128 scanned nodes gave a sensitivity of 69% for detection of clinically relevant metastases (71% for macrometastases) and a specificity of 96%, comparable to literature results for touch imprint cytology, a standard technique for intra-operative diagnosis. ESS has the advantage of not requiring a pathologist to review the tissue sample. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3463005]

引用

页数：8

共 24 条

[1] Noninvasive measurement of oxygen saturation of the microvascular blood in Barrett's dysplasia by use of optical spectroscopy [J].