Surface-Sensitive Imaging Analysis of Cell-Microenvironment Interactions by Electrochemiluminescence Microscopy

被引:16
作者
Ding, Lurong [1 ]
Ding, Hao [1 ]
Zhou, Ping [1 ]
Xi, Lingling [1 ]
Su, Bin [1 ]
机构
[1] Zhejiang Univ, Dept Chem, Key Lab Excited State Mat Zhejiang Prov, Hangzhou 310000, Peoples R China
基金
中国国家自然科学基金;
关键词
NEURITE OUTGROWTH; ALPHA-1-BETA-1; INTEGRINS; ADHESION; RGD;
D O I
10.1021/acs.analchem.2c02479
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A complex and heterogeneous cell microenvironment offers not only structural support for cells but also myriad biochemical and biophysical cues. These outside-in signals transmit into cells primarily through integrins, which are the important components of cell-matrix adhesions to direct and maintain cell behaviors and fate. In this work, we report a surface-sensitive imaging methodology for evaluating the difference in cell-matrix adhesions at the single cell level to dissect the impact of the chemical microenvironment on cell behaviors. Cells were cultured on silica nanochannel membrane (SNM) modified indium tin oxide (ITO) electrodes (SNM/ITO) with different terminal surfaces and imaged by electrochemiluminescence microscopy (ECLM). The results show that the surface tethered with Arg-Gly-Asp (RGD) groups can mediate robust cell-microenvironment interaction and those coated with silanol and (3-aminopropyl)triethoxysilane (APTES) groups transmit an intermediate adhesion, while oligo(ethylene glycol) (OEG) coated surface conveys the weakest cell-matrix adhesion. Specific recognition of integrins to different surfaces was further explored in conjunction with selective immunoblocking of different subunits. alpha 6, alpha 5, and alpha 1 integrin subunits were found to recognize SNM, RGD/OEG, and APTES surfaces, respectively. The work provides not only insights into cell-microenvironment interaction but also guideline in the design and development of functional and biomimetic surface materials.
引用
收藏
页码:10885 / 10892
页数:8
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