Evaluation of DNA binding and DNA cleavage of nickel(II) complexes with tridentate α-N-heterocyclic thiosemicarbazones ligands

被引:29
作者
Deng, JunGang [1 ]
Su, Gaoxing [3 ]
Chen, Peng [3 ]
Du, Yunpeng [1 ]
Gou, Yi [3 ]
Liu, Yani [1 ,2 ]
机构
[1] Guilin Med Coll, Affiliated Hosp, Guilin, Guangxi, Peoples R China
[2] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Pharm, Wuhan, Hubei, Peoples R China
[3] Nantong Univ, Sch Pharm, Nantong, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Thiosemicarbazones; Nickel(II) complexes; DFT calculations; DNA interactions; IN-VITRO; NI(II) COMPLEXES; IRON CHELATORS; BIOLOGICAL-PROPERTIES; COPPER(II) COMPLEXES; CRYSTAL-STRUCTURE; NUCLEASE ACTIVITY; ZN(II) COMPLEXES; DNA/BSA BINDING; METAL-COMPLEXES;
D O I
10.1016/j.ica.2017.11.013
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
Two mononuclear thiosemicarbazones nickel(II) complexes of the type [Ni(L1)(2)] (1) and [Ni(HL2)(L2)] (2), where HL1 = (E)-N-methyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide and HL2 = (E)-N, N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamidehave been synthesized and characterized by X-ray crystallography, infrared spectroscopy, elemental analysis and DFT calculations. Single-crystal diffraction analysis revealed that the HL1 ligands in 1 are predominantly in the thiolato resonance forms, while the HL2 ligands in 2 coordinate in thiolato and thione forms simultaneously. The interactions of both nickel(II) complexes with calf thymus DNA (CT-DNA) and supercoiled pBR322 plasmid DNA were investigated by fluorescence and UV-visible spectra, viscosity measurement, as well as agarose gel electrophoresis. The results of binding experiments displayed that both nickel(II) complexes were able to interact with CT-DNA via intercalative mode with a moderate binding affinity in the order 2 > 1. Gel electrophoresis assay revealed that the complexes could cleave the pBR322 plasmid DNA in a dose-dependent manner. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:194 / 202
页数:9
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