Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human ß-galactosidase

被引：2

作者：

Usui, Kimihito ^{[1
]}

Ohto, Umeharu ^{[1
]}

Ochi, Toshinari ^{[1
]}

Shimizu, Toshiyuki ^{[1
]}

Satow, Yoshinori ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2012年 / 68卷

关键词：

ss-galactosidase; galactose; 1-deoxygalactonojirimycin; HUMAN BETA-GALACTOSIDASE; GENE-MUTATIONS; GM1-GANGLIOSIDOSIS; PROTEIN; PRECURSOR; CRYSTAL; DISEASE; CDNA;

D O I：

10.1107/S1744309111047920

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-d-Galactosidase (beta-Gal) is an exoglycosidase that cleaves beta-galactosides from glycoproteins, sphingolipids and keratan sulfate. This study reports the expression, purification, crystallization and preliminary X-ray crystallographic analysis of human lysosomal beta-Gal. The sitting-drop vapour-diffusion method was used to crystallize beta-Gal in complexes with its product galactose and with the inhibitor 1-deoxygalactonojirimycin. The resulting crystals were isomorphous and belonged to space group P2(1). The crystals of the beta-Galgalactose and the beta-Galinhibitor complexes had unit-cell parameters a = 94.8, b = 116.1, c = 140.3 angstrom, beta = 92.2 degrees and a = 94.8, b = 116.0, c = 140.3 angstrom, beta = 92.2 degrees, respectively. Diffraction data were collected to 1.8 angstrom resolution for both crystals.

引用

页码：73 / 77

页数：5

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