Nanostructured Immunosensor for Attomolar Detection of Cancer Biomarker Interleukin-8 Using Massively Labeled Superparamagnetic Particles

被引：142

作者：

Munge, Bernard S. ^{[1
]}

Coffey, Amy L. ^{[1
]}

Doucette, Jaimee M. ^{[1
]}

Somba, Brian K. ^{[1
]}

Malhotra, Ruchika ^{[2
,3
]}

Patel, Vyomesh ^{[4
]}

Gutkind, J. Silvio ^{[4
]}

Rusling, James F. ^{[2
,3
]}

机构：

[1] Salve Regina Univ, Dept Chem, Newport, RI 02840 USA

[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[3] Univ Connecticut, Ctr Hlth, Dept Cell Biol, Farmington, CT 06232 USA

[4] NIDCR, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 34期

关键词：

cancer; electrochemistry; immunosensors; interleukin-8; magnetic beads; PROSTATE-SPECIFIC ANTIGEN; CARBON NANOTUBE FOREST; GROWTH-FACTOR; HEAD; MICROARRAY; MARKERS; STRATEGIES; ELECTRODES; PROTEINS; SENSOR;

D O I：

10.1002/anie.201102941

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A sensitive issue: Superparamagnetic particle-antibody conjugates enable detection of interleukin-8 (IL-8) in serum at a level of 1 fgmL -1 (ca. 100 aM). Ultrahigh sensitivity is facilitated by a nanostructured sensor platform coupled with secondary antibody-magnetic bead-horseradish peroxidase conjugate (Ab 2-MB-HRP) with about 500000 HRP labels (see picture). © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7915 / 7918

页数：4

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