Mimicking tumor hypoxia and tumor-immune interactions employing three-dimensional in vitro models

被引:69
作者
Bhattacharya, Somshuvra [1 ]
Calar, Kristin [1 ]
de la Puente, Pilar [1 ,2 ,3 ]
机构
[1] Sanford Res, Canc Biol & Immunotherapies Grp, 2301 E 60th St N, Sioux Falls, SD 57104 USA
[2] Univ South Dakota, Dept Surg, Sanford Sch Med, Sioux Falls, SD 57105 USA
[3] South Dakota State Univ, Dept Chem & Biochem, Brookings, SD 57007 USA
基金
美国国家卫生研究院;
关键词
Tumor microenvironment; Three-dimensional; In vitro models; Hypoxia; Immune; Bioengineering; Cancer; EXTRACELLULAR-MATRIX; CANCER ORGANOIDS; DRUG-RESISTANCE; CELL-CULTURE; BONE-MARROW; HUMAN LIVER; 3D MODEL; MICROENVIRONMENT; HIF-1-ALPHA; GRADIENTS;
D O I
10.1186/s13046-020-01583-1
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The heterogeneous tumor microenvironment (TME) is highly complex and not entirely understood. These complex configurations lead to the generation of oxygen-deprived conditions within the tumor niche, which modulate several intrinsic TME elements to promote immunosuppressive outcomes. Decoding these communications is necessary for designing effective therapeutic strategies that can effectively reduce tumor-associated chemotherapy resistance by employing the inherent potential of the immune system. While classic two-dimensional in vitro research models reveal critical hypoxia-driven biochemical cues, three-dimensional (3D) cell culture models more accurately replicate the TME-immune manifestations. In this study, we review various 3D cell culture models currently being utilized to foster an oxygen-deprived TME, those that assess the dynamics associated with TME-immune cell penetrability within the tumor-like spatial structure, and discuss state of the art 3D systems that attempt recreating hypoxia-driven TME-immune outcomes. We also highlight the importance of integrating various hallmarks, which collectively might influence the functionality of these 3D models. This review strives to supplement perspectives to the quickly-evolving discipline that endeavors to mimic tumor hypoxia and tumor-immune interactions using 3D in vitro models.
引用
收藏
页数:16
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