Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

被引：9

作者：

Tian, Zeyu ^{[1
]}

Wang, Chun-Ko ^{[2
]}

Lin, Feng-Ling ^{[3
]}

Liu, Qian ^{[1
]}

Wang, Ting ^{[1
]}

Sung, Tzu-Cheng ^{[1
]}

Alarfaj, Abdullah A. ^{[4
]}

Hirad, Abdurahman H. ^{[4
]}

Lee, Henry Hsin-Chung ^{[5
,6
,7
]}

Wu, Gwo-Jang ^{[8
,9
]}

Higuchi, Akon ^{[1
,2
,10
,11
]}

机构：

[1] Wenzhou Med Univ, Sch Ophthalmol & Optometry, Eye Hosp, 270 Xueyuan Rd, Wenzhou 325027, Zhejiang, Peoples R China

[2] Natl Cent Univ, Dept Chem & Mat Engn, 300 Jhongda RD, Taoyuan 300, Taiwan

[3] Cathay Gen Hosp, Dept Dermatol, Taipei 22174, Taiwan

[4] King Saud Univ, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia

[5] Cathay Gen Hosp, Dept Surg, Taipei 10630, Taiwan

[6] Sijhih Cathay Gen Hosp, Dept Surg, New Taipei 22174, Taiwan

[7] Natl Cent Univ, Grad Inst Translat & Interdisciplinary Med, 300 Jhongda RD, Taoyuan 300, Taiwan

[8] Natl Def Med Ctr, Triserv Gen Hosp, Grad Inst Med Sci, Taipei 11490, Taiwan

[9] Natl Def Med Ctr, Triserv Gen Hosp, Dept Obstet & Gynecol, Taipei 11490, Taiwan

[10] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Taoyuan 320, Taiwan

[11] Natl Ctr Child Hlth & Dev, Dept Reprod, Setagaya Ku, 2-10-1 Okura, Tokyo 1578535, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2022年 / 10卷 / 30期

关键词：

PHYSICAL CUES; BIOMATERIALS; CULTURE; ELASTICITY;

D O I：

10.1039/d2tb01026g

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

The transplantation of human mesenchymal stem cells (hMSCs), such as bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs), has shown beneficial effects in protecting transplanted tissues and cells against graft-versus-host disease (GVHD). Human pluripotent stem cell (hPSC)-derived mesenchymal stem cells (MSCs) can also be used to generate hMSCs with stable characteristics without limitations. Therefore, we differentiated human induced pluripotent stem cells (hiPSCs, H-M5) and human embryonic stem cells (hESCs, H9) into hMSCs on dishes coated with different extracellular matrix (ECM) proteins to study the effect of cell culture biomaterials on hPSC differentiation into hMSCs. hPSC-derived MSCs cultured on Matrigel (MAT)-coated, collagen (COL)-coated and laminin-521 (LN-521)-coated tissue culture polystyrene (TCP) dishes showed excellent proliferation speed and reduced aging over 10 passages. High MSC surface marker (CD44, CD73, CD90 and CD105) expression was also observed on hPSC-derived MSCs cultured on MAT-coated, COL-coated and LN-521-coated TCP dishes as well as uncoated TCP dishes. Analysis of late osteogenic differentiation by evaluation of mineral deposition revealed that hPSC-derived MSCs cultured on fibronectin (FN)-coated and LN-521-coated TCP dishes showed high osteogenic differentiation. ECM proteins are effective as coating materials on cell culture biomaterials to regulate the proliferation and differentiation fate of hPSC-derived MSCs.

引用

页码：5723 / 5732

页数：11

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