Protection by exogenous pyruvate through a mechanism related to monocarboxylate transporters against cell death induced by hydrogen peroxide in cultured rat cortical neurons

被引：42

作者：

Nakamichi, N ^{[1
]}

Kambe, Y ^{[1
]}

Oikawa, H ^{[1
]}

Ogura, M ^{[1
]}

Takano, K ^{[1
]}

Tamaki, K ^{[1
]}

Inoue, M ^{[1
]}

Hinoi, E ^{[1
]}

Yoneda, Y ^{[1
]}

机构：

[1] Kanazawa Univ, Grad Sch Nat Sci & Technol, Div Pharmaceut Sci, Mol Pharmacol Lab, Kanazawa, Ishikawa 9201192, Japan

来源：

JOURNAL OF NEUROCHEMISTRY | 2005年 / 93卷 / 01期

关键词：

hydrogen peroxide; monocarboxylate transporters; neuroprotection; neurotoxicity; pyruvate;

D O I：

10.1111/j.1471-4159.2005.02999.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In cortical neurons cultured for 3 or 9 days in vitro (DIV), exposure to hydrogen peroxide (H2O2) led to a marked decrease in cell viability in a concentration-dependent manner at a concentration range of 10 mu M to 1 mM irrespective of the duration between 6 and 24 h. However, H2O2 was more potent in decreasing cellular viability in cortical neurons cultured for 9 DIV than in those for 3 DIV. Pyruvate was effective in preventing the neuronal cell death at 1 mM even when added 1-3 h after the addition of H2O2. Semi-quantitative RT-PCR and western blotting analyses revealed significantly higher expression of both mRNA and protein for a particular monocarboxylate transporter (MCT) in neurons cultured for 9 DIV than in those for 3 DIV. A specific inhibitor of MCT significantly attenuated the neuroprotection by pyruvate in neurons cultured for 9 DIV, without markedly affecting that in neurons cultured for 3 DIV. These results suggest that vulnerability to H2O2 may at least in part involve expression of particular MCT isoforms responsible for the bi-directional transport of pyruvate across cell surfaces in cultured rat cortical neurons.

引用

页码：84 / 93

页数：10

共 33 条

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