Potent immune response against HIV-1 and protection from virus challenge in hu-PBL-SCID mice immunized with inactivated virus-pulsed dendritic cells generated in the presence of IFN-α

被引：103

作者：

Lapenta, C ^{[1
]}

Santini, SM ^{[1
]}

Logozzi, M ^{[1
]}

Spada, M ^{[1
]}

Andreotti, M ^{[1
]}

Di Pucchio, T ^{[1
]}

Parlato, S ^{[1
]}

Belardelli, F ^{[1
]}

机构：

[1] Ist Super Sanita, Virol Lab, I-00161 Rome, Italy

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 2003年 / 198卷 / 02期

关键词：

antigen presenting cell; vaccine; neutralizing antibodies; CD8(+) T lymphocytes; AIDS;

D O I：

10.1084/jem.20021924

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A major challenge of AIDS research is the development of therapeutic vaccine strategies capable of inducing the Immoral and cellular arms of the immune responses against HIV-1. In this work, we evaluated the capability of DCs pulsed with aldrithiol-2-inactivated HIV-1 in inducing a protective antiviral human immune response in SCID mice reconstituted with human PBL (hu-PBL-SCID mice). Immunization of hu-PBL-SCID mice with DCs generated after exposure of monocytes to GM-CSF/IFN-alpha (IFN-DCs) and pulsed with inactivated HIV-1 resulted in a marked induction of human anti-HIV-1 antibodies, which was associated with the detection of anti-HIV neutralizing activity in the serum. This vaccination schedule also promoted the generation of a human CD8(+) T cell response against HIV-1, as measured by IFN-gamma Elispot analysis. Notably, when the hu-PBL-SCID mice immunized with antigen-pulsed IFN-DCs were infected with HIV-1, inhibition of virus infection was observed as compared with control animals. These results suggest that IFN-DCs pulsed with inactivated HIV-1. can represent a valuable approach of immune intervention in HIV-1-infected patients.

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页码：361 / 367

页数：7

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