Large-scale analysis of HLA peptides presented by HLA-Cw4

被引:18
作者
Buchsbaum, S
Barnea, E
Dassau, L
Beer, I
Milner, E
Admon, A [1 ]
机构
[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel
[2] IBM Res Lab, Haifa, Israel
关键词
cancer antigens; HLA-Cw4; peptide binding specificity;
D O I
10.1007/s00251-003-0570-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A large number of HLA-Cw4 (Cw *0402) peptides were purified, sequenced, and identified from breast and ovarian carcinoma cell lines. HLA-Cw4 molecules were expressed in these cells as soluble, secreted HLA (sHLA) and recovered from the growth medium. The peptides were separated by capillary reversed-phase HPLC and analyzed by tandem mass-spectrometry. The resulting peptides fit to some extent, but not completely, the known consensus of the Cw4 peptide-binding motif. Among the identified peptides, there are a few that originate from proteins of possible interest for cancer immunotherapy or diagnostics, including mucin-5B, ART-1, fatty acid synthase, putative prostate cancer tumor suppressor, DNA topoisomerase-1, and Rac1. This work demonstrates that large-scale identification of HLA peptides recovered from sHLA is an advantageous approach for establishing the HLA peptide consensus of different haplotypes and the identification of useful peptides for treatment of diseases such as cancer, viral, and autoimmune diseases.
引用
收藏
页码:172 / 176
页数:5
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