Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam

被引：35
作者：
Shao, Wenyong [1 ]
Zhang, Yu [1 ]
Ren, Weichao [1 ]
Chen, Changjun [1 ]
机构：
[1] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
来源：
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2015年 / 117卷
基金：
中国国家自然科学基金;
关键词：
Botrytis cinerea; Fluazinam; Resistance; Characteristics; SCLEROTINIA-SCLEROTIORUM; STRAWBERRY; FUNGICIDES; PHENYLPYRROLE; CROPS; FLUDIOXONIL; MANAGEMENT; CLUBROOT; MOLD;
D O I：
10.1016/j.pestbp.2014.10.003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Botrytis cinerea is a necrotrophic and filamentous fungus with a high risk of developing resistance to fungicides. The pyridinamine fungicide fluazinam has been reported to have excellent activity against B. cinerea and better effect on controlling gray mold. In this study, the physiological and biochemical characteristics of laboratory-induced mutants of B. cinerea with resistance to fluazinam has been investigated. Compared to the wild-type strains, the fluazinam-resistant mutants had a significant decrease in respiratory rate, glycerol, oxalate, and ATP contents, and an increase in ATPase activity and sensitivity to osmotic pressure, but did not differ in cell membrane permeability. Sequencing indicated that two parental strains and four resistant mutants were identical in the nucleotide sequence of F-ATPase gene. These results will enrich our understanding of the resistance mechanism of B. cinerea to fluazinam. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：19 / 23
页数：5
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