Epithelial-mesenchymal transition transcription factors control pluripotent adult stem cell migration in vivo in planarians

被引：47
作者：
Abnave, Prasad [1 ]
Aboukhatwa, Ellen [1 ]
Kosaka, Nobuyoshi [1 ]
Thompson, James [2 ]
Hill, Mark A. [2 ]
Aboobaker, A. Aziz [1 ]
机构：
[1] Univ Oxford, Dept Zool, Tinbergen Bldg,South Parks Rd, Oxford OX1 3PS, England
[2] Univ Oxford, CRUK, MRC, Oxford Inst Radiat Oncol, ORCRB Roosevelt Dr, Oxford OX3 7DQ, England
来源：
DEVELOPMENT | 2017年 / 144卷 / 19期
基金：
英国生物技术与生命科学研究理事会; 英国医学研究理事会;
关键词：
EMT; Migration; Planarian; Pluripotency; Snail; Wounding; Schmidtea mediterranea; SCHMIDTEA-MEDITERRANEA; FACTOR SNAIL; HEPATOCELLULAR-CARCINOMA; REGENERATING PLANARIANS; CANCER INVASION; GASTRIC-CANCER; EXPRESSION; TISSUE; PROGRESSION; NOTUM;
D O I：
10.1242/dev.154971
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Migration of stem cells underpins the physiology of metazoan animals. For tissues to be maintained, stem cells and their progeny must migrate and differentiate in the correct positions. This need is even more acute after tissue damage by wounding or pathogenic infection. Inappropriate migration also underpins metastasis. Despite this, few mechanistic studies address stem cell migration during repair or homeostasis in adult tissues. Here, we present a shielded X-ray irradiation assay that allows us to follow stem cell migration in planarians. We demonstrate the use of this system to study the molecular control of stem cell migration and show that snail-1, snail-2 and zeb-1 EMT transcription factor homologs are necessary for cell migration to wound sites and for the establishment of migratory cell morphology. We also observed that stem cells undergo homeostatic migration to anterior regions that lack local stem cells, in the absence of injury, maintaining tissue homeostasis. This requires the polarity determinant notum. Our work establishes planarians as a suitable model for further in-depth study of the processes controlling stem cell migration in vivo.
引用
收藏
页码：3440 / 3453
页数：14
相关论文
共 71 条
[1]   Inhibition of Epithelial to Mesenchymal Transition by E-cadherin Up-regulation via Repression of Slug Transcription and Inhibition of E-cadherin Degradation DUAL ROLE OF SCAFFOLD/MATRIX ATTACHMENT REGION-BINDING PROTEIN 1 (SMAR1) IN BREAST CANCER CELLS [J].
Adhikary, Arghya ;
Chakraborty, Samik ;
Mazumdar, Minakshi ;
Ghosh, Swatilekha ;
Mukherjee, Shravanti ;
Manna, Argha ;
Mohanty, Suchismita ;
Nakka, Kiran Kumar ;
Joshi, Shruti ;
De, Abhijit ;
Chattopadhyay, Samit ;
Sa, Gaurisankar ;
Das, Tanya .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (37) :25431-25444
[2]   The inhibitive action of the Roentgen rays on regeneration in planarians [J].
Bardeen, CR ;
Baetjer, FH .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1904, 1 (01) :191-195
[3]   The transcription factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells [J].
Batlle, E ;
Sancho, E ;
Franci, C ;
Domínguez, D ;
Monfar, M ;
Baulida, J ;
de Herreros, AG .
NATURE CELL BIOLOGY, 2000, 2 (02) :84-89
[4]   Snail levels control the migration mechanism of mesenchymal tumor cells [J].
Belgiovine, Cristina ;
Chiesa, Giulio ;
Chiodi, Ilaria ;
Frapolli, Roberta ;
Bonezzi, Katiuscia ;
Taraboletti, Giulia ;
D'Incalci, Maurizio ;
Mondello, Chiara .
ONCOLOGY LETTERS, 2016, 12 (01) :767-771
[5]   Correlation of Snail expression with histological grade and lymph node status in breast carcinomas [J].
Blanco, MJ ;
Moreno-Bueno, G ;
Sarrio, D ;
Locascio, A ;
Cano, A ;
Palacios, J ;
Nieto, MA .
ONCOGENE, 2002, 21 (20) :3241-3246
[6]   Integrin suppresses neurogenesis and regulates brain tissue assembly in planarian regeneration [J].
Bonar, Nicolle A. ;
Petersen, Christian P. .
DEVELOPMENT, 2017, 144 (05) :784-794
[7]   Distinct mechanisms underlie oral vs aboral regeneration in the cnidarian Hydractinia echinata [J].
Bradshaw, Brian ;
Thompson, Kerry ;
Frank, Uri .
ELIFE, 2015, 4 :1-19
[8]   The transcription factor Snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression [J].
Cano, A ;
Pérez-Moreno, MA ;
Rodrigo, I ;
Locascio, A ;
Blanco, MJ ;
del Barrio, MG ;
Portillo, F ;
Nieto, MA .
NATURE CELL BIOLOGY, 2000, 2 (02) :76-83
[9]   HOX gene complement and expression in the planarian Schmidtea mediterranea [J].
Currie, Ko W. ;
Brown, David D. R. ;
Zhu, Shujun ;
Xu, ChangJiang ;
Voisin, Veronique ;
Bader, Gary D. ;
Pearson, Bret J. .
EVODEVO, 2016, 7
[10]   Epithelial mesenchymal transition in ovarian carcinoma [J].
Davidson, Ben ;
Trope, Claes G. ;
Reich, Reuven .
FRONTIERS IN ONCOLOGY, 2012, 2
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