Adipocyte fatty acid-binding protein: An important gene related to lipid metabolism in chicken adipocytes

被引：19

作者：

Shi, Hui ^{[1
]}

Wang, Qigui ^{[1
]}

Wang, Yuxiang ^{[1
]}

Leng, Li ^{[1
]}

Zhang, Qingqiu ^{[1
]}

Shang, Zhouchun ^{[1
]}

Li, Hui ^{[1
]}

机构：

[1] NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Heilongjiang, Peoples R China

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2010年 / 157卷 / 04期

关键词：

A-FABP; Lipid accumulation; Lipolysis; Chicken; IN-VIVO; LIPOLYSIS; TISSUE; MICE; AP2; OBESITY; CELLS;

D O I：

10.1016/j.cbpb.2010.08.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fatty acid-binding proteins (FABPs) may facilitate the diffusion of fatty acids within the cytoplasm. Adipocyte FABP (A-FABP) null mice exhibit altered rates of lipolysis, and reprogrammed expression of genes related to lipid metabolism. In the current study, chicken adipocytes with short hairpin RNA-mediated knockdown and cDNA overexpression of A-FABP were used to investigate the role of chicken A-FABP in lipid metabolism of adipocytes. The results showed that there was no significant change of lipid metabolism in the A-FABP interference adipocytes. However, in the A-FABP overexpression adipocytes, the lipolysis was increased significantly at 36 h (p<0.05), and lipid accumulation was increased significantly at 48, 60 and 72 h (p<0.05). The expression of PPAR gamma and perilipin genes were upregulated, while the expression of E-FABP gene was downregulated after transfection with pcDNA3.1/A-FABP (p<0.05). The present study suggested that A-FABP gene might affect the lipid metabolism through the PPAR gamma pathway, and the function between A-FABP and E-FABP gene was compensatory in the chickens. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：357 / 363

页数：7

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