Fungi allergens produced by solid-state fermentation process

被引：0

作者：

Hasan, SDM

Gambale, W

Zollner, RL

Santana, MHA

机构：

[1] Univ Estadual Campinas, Sch Chem Engn, BR-13083970 Campinas, SP, Brazil

[2] Univ Sao Paulo, Lab Mycol, Inst Biomed Sci, BR-05508900 Sao Paulo, Brazil

[3] Univ Estadual Campinas, Sch Med Sci, BR-13083970 Campinas, SP, Brazil

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2003年 / 105卷

关键词：

allergenic extract; Drechslera (Helminthosporium) monoceras; solid-state fermentation; statistical experimental design; wheat bran; proteins; biomass;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate . d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays.

引用

页码：403 / 412

页数：10

共 16 条

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