Cytokine stimulation of the choriocarcinoma cell line JEG-3 leads to alterations in the HLA-G expression profile

被引：22

作者：

Persson, Gry ^{[1
,2
]}

Bork, Julie Birgit Siig ^{[1
,2
]}

Isgaard, Cecilie ^{[1
,2
]}

Larsen, Tine Graakjaer ^{[1
,2
]}

Bordoy, Anna Maria ^{[1
,2
]}

Bengtsson, Meghan Sand ^{[1
,2
]}

Faurschou, Thomas Vauvert ^{[1
,2
]}

机构：

[1] Zealand Univ Hosp, Dept Clin Biochem, ReproHlth Consortium ZUH, Ctr Immune Regulat & Reprod Immunol CIRRI, 10 Sygehusvej, DK-4000 Roskilde, Denmark

[2] Univ Copenhagen, Dept Clin Med, 10 Sygehusvej, DK-4000 Roskilde, Denmark

来源：

CELLULAR IMMUNOLOGY | 2020年 / 352卷

关键词：

Cytokine; Immune tolerance; Pregnancy; Preeclampsia; Cancer; Cell line; HLA class Ib; HLA-G; Isoform; ANTIGEN-PRESENTING CELLS; G MESSENGER-RNA; G ISOFORMS; CLASS-I; EXTRAVILLOUS TROPHOBLASTS; DIFFERENTIAL REGULATION; INHIBITORY RECEPTOR; SOLUBLE HLA-G1; ALPHA-1; DOMAIN; T-CELL;

D O I：

10.1016/j.cellimm.2020.104110

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The checkpoint molecule human leukocyte antigen (HLA)-G has restricted tissue expression, and plays a role in the establishment of maternal tolerance to the semi-allogenic fetus during pregnancy by expression on the trophoblast cells in the placenta. HLA-G exists in at least seven well-described mRNA isoforms, of which four are membrane-bound and three soluble. Regulation of the tissue expression of HLA-G and its isoforms is relatively unknown. Therefore, it is important to understand the regulation of HLA-G, and the HLA-G(+) choriocarcinoma cell line JEG-3 is a widely used cellular model. We hypothesized that cytokines present in the microenvironment can regulate the HLA-G expression profile. In the present study, we systematically stimulated JEG-3 cells with various concentrations of IL-2, IL-4 IL-6, IL-10, IL-12, IL-15, IL-17A, TGF-beta(1), TNF-alpha and IFN-gamma 1b. The results suggest that IFN-. plays a role in maintenance of HLA-G expression, while IL-10 might be involved in regulation of the isoform profile.

引用

页数：15

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