Plastin 1 promotes osteoblast differentiation by regulating intracellular Ca2+

被引:10
作者
Wang, Lianqing [1 ,2 ]
Lan, Yongting [3 ]
Du, Yanqin [4 ]
Xiang, Xinxin [1 ]
Tian, Wenxiu [1 ]
Yang, Baoye [1 ]
Li, Tao [1 ]
Zhai, Qiaoli [1 ]
机构
[1] Shandong Univ, Zibo Cent Hosp, Ctr Translat Med, Zibo 255036, Peoples R China
[2] Chinese Acad Med Sci, Sch Basic Med, McKusick Zhang Ctr Genet Med, Inst Basic Med Sci,Peking Union Med Coll, Beijing 100005, Peoples R China
[3] Shandong Univ, Zibo Cent Hosp, Div Gastroenterol, Zibo 255036, Peoples R China
[4] Peking Univ, Dept Gynecol, Care Luzhong Hosp, Zibo 255400, Peoples R China
基金
中国国家自然科学基金;
关键词
plastin; 1; osteoblast differentiation; intracellular Ca2+; 3; osteoporosis; OSTEOPOROSIS; EXPRESSION; MUTATION;
D O I
10.1093/abbs/gmaa027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteoblast differentiation is a key process in bone homeostasis. Mutations in plastin 3 have been reported to be responsible for X-linked osteoporosis. Plastin 3 and plastin 2 act synergistically to regulate osteoblast differentiation. However, the bone-related function of plastin 1, another family member of plastins, has not been assessed. In this study, we addressed the functional importance of plastin 1 in osteoblasts. We characterized the expression patterns of plastin 1 during osteoblast differentiation and revealed its important role in this process. In both HEK 293T and hFOB1.19 cells, plastin 1 was demonstrated to regulate intracellular Ca2+. Accordingly, we revealed that higher Ca2+ concentration promotes osteoblast differentiation. Finally, we found that plastin 1 may play a compensatory role in osteoporosis patients with plastin 3 deficiency. Together, our results indicate that plastin 1 promotes osteoblast differentiation by regulating intracellular Ca2+. Our work sheds new light on the role played by plastins in bone homeostasis.
引用
收藏
页码:563 / 569
页数:7
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