Assessing the Sporicidal Activity of Oligo-p-phenylene Ethynylenes and Their Role as Bacillus Germinants

被引:15
作者
Pappas, Harry C. [1 ,2 ]
Lovchik, Julie A. [3 ]
Whitten, David G. [2 ]
机构
[1] 1 Univ New Mexico, Nanosci & Microsyst Engn Program, Univ New Mexico, Albuquerque, NM 87131 USA
[2] 1 Univ New Mexico, Ctr Biomed Engn, Univ New Mexico, Dept Chem & Biol Engn, Albuquerque, NM 87131 USA
[3] 1 Univ New Mexico, Hlth Sci Ctr, Univ New Mexico, Dept Internal Med, Albuquerque, NM 87131 USA
关键词
CATIONIC CONJUGATED POLYELECTROLYTES; BIOCIDAL ACTIVITY; SUBTILIS SPORES; BACTERIAL; LIGHT; MECHANISMS; DARK; TEMPERATURE; RESISTANCE; ANTHRACIS;
D O I
10.1021/acs.langmuir.5b00064
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A wide range of oligo-p-phenylene ethynylenes has been shown to exhibit good biocidal activity against both Gram-negative and Gram-positive bacteria. While cell death may occur in the dark, these biocidal compounds are far more effective in the light as a result of their ability to sensitize the production of cell-damaging reactive oxygen species. In these studies, the interactions of a specific cationic oligo-p-phenylene ethynylene with spore-forming Bacillus atrophaeus and Bacillus anthracis Sterne have been investigated. Flow cytometry assays are used to rapidly monitor cell death as well as spore germination. This compound effectively killed Bacillus anthracis Sterne vegetative cells (over 4 log reduction), presumably by severe perturbations of the bacterial cell wall and cytoplasmic membrane, while also acting as an effective spore germinant in the dark. While 2 log reduction of B. anthracis Sterne spores was observed, it is hypothesized that further killing could be achieved through enhanced germination.
引用
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页码:4481 / 4489
页数:9
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