Callicarpa japonica Thunb. reduces inflammatory responses: A mouse model of lipopolysaccharide-induced acute lung injury

被引：25

作者：

Shin, Na-Rae ^{[1
,2
]}

Shin, In-Sik ^{[1
,3
]}

Song, Hyuk-Hwan ^{[1
]}

Hong, Ju-Mi ^{[1
]}

Kwon, Ok-Kyoung ^{[1
]}

Jeon, Chan-Mi ^{[1
]}

Kim, Jung-Hee ^{[1
]}

Lee, Sang-Woo ^{[4
]}

Lee, Joong-Ku ^{[4
]}

Jin, Hang ^{[5
]}

Li, Wan Yi ^{[5
]}

Oh, Sei-Ryang ^{[1
]}

Hahn, Kyu-Woung ^{[2
]}

Ahn, Kyung-Seop ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Nat Med Res Ctr, Chungju Si 363883, Chungbuk, South Korea

[2] Hannam Univ, Taejon 305811, South Korea

[3] Chonnam Natl Univ, Coll Vet Med, Kwangju 500757, South Korea

[4] Korea Res Inst Biosci & Biosci & Biotech, Int Biol Mat Res Ctr, Taejon 305806, South Korea

[5] Yunnan Acad Agr Sci, Med Plant Res Inst, Yunnan, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2015年 / 26卷 / 01期

关键词：

Callicarpa japonica Thunb; Acute lung injury; Inducible nitric oxide synthase; Interleukin-6; NITRIC-OXIDE SYNTHASE; NF-KAPPA-B; REACTIVE OXYGEN; AIRWAY INFLAMMATION; DOWN-REGULATION; MICE; ACTIVATION; EXTRACT; SUPPRESSION; EXPRESSION;

D O I：

10.1016/j.intimp.2015.01.025

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Callicarpa japonica Thunb. (CJT) is traditionally used as an herbal remedy for the treatment of inflammatory diseases. This study aimed to investigate the anti-inflammatory response of CJT in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and LPS-induced acute lung injury (ALL) in mice. The C57BL/6 mice were administered 30 mg/kg of CJT by oral gavage for 3 days. LPS is applied to animals by intranasal administration 1 h after final CJT treatment. LPS is applied to animals by intranasal administration 1 h after final CJT treatment LPS was delivered intranasally 1 h after the final CJT treatment In the LPS-stimulated RAW264.7 cells, CJT significantly decreased nitric oxide (NO) and interleukin (IL)-6 in a concentration-dependent manner by reducing inducible NO synthase (iNOS) and IL-6 mRNA levels. In the ALI model, CJT decreased the inflammatory cell count in the bronchoalveolar lavage fluid (BALF) while IL-6 levels were reduced in CJT-treated mice compared with the ALI control mice. CJT also inhibited airway inflammation by reducing iNOS expression in lung tissue. In conclusion, our results indicate that CJT inhibits inflammatory responses in LPS-stimulated RAW264.7 cells and in the LPS-induced ALI model. Therefore, we suggest that CJT has the potential to treat inflammatory diseases such as pneumonia. (c) 2015 Elsevier B.V. All rights reserved.

引用

页码：174 / 180

页数：7

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