The genomic structure of the gene encoding the human transforming growth factor beta type II receptor (TGF-beta RII)

被引：38

作者：

Takenoshita, S ^{[1
]}

Hagiwara, K ^{[1
]}

Nagashima, M ^{[1
]}

Gemma, A ^{[1
]}

Bennett, WP ^{[1
]}

Harris, CC ^{[1
]}

机构：

[1] NCI,HUMAN CARCINOGENESIS LAB,DIV BASIC SCI,NIH,BETHESDA,MD 20892

来源：

GENOMICS | 1996年 / 36卷 / 02期

关键词：

D O I：

10.1006/geno.1996.0471

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genomic structure of the human transforming growth factor-beta type II receptor gene (TGF-beta RII) was determined by two PCR-based methods, the ''long distance sequencer'' method and the ''promoter finder'' method. Genomic fragments containing exons and adjacent introns were amplified by PCR, and the nucleotide sequences were determined by direct sequencing and subcloning sequencing. The TGF-beta RII protein is encoded by 567 codons in 7 exons, This is the first report about the genomic structure of a gene that belongs to the serine/threonine kinase type II receptor subfamily. Knowledge of the genomic structure of the TGF-beta RII gene will facilitate investigation of the TGF-beta signaling pathway in normal human cells and of the aberrations occurring during carcinogenesis. (C) 1996 Academic Press, Inc.

引用

页码：341 / 344

页数：4

共 18 条

[1] CHARACTERIZATION OF THE PROMOTER REGION OF THE HUMAN TRANSFORMING GROWTH-FACTOR-BETA TYPE-II RECEPTOR GENE [J].

BAE, HW ;

GEISER, AG ;

KIM, DH ;

CHUNG, MT ;

BURMESTER, JK ;

SPORN, MB ;

ROBERTS, AB ;

KIM, SJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) :29460-29468

[2] A WD-DOMAIN PROTEIN THAT IS ASSOCIATED WITH AND PHOSPHORYLATED BY THE TYPE-II TGF-BETA RECEPTOR [J].

CHEN, RH ;

MIETTINEN, PJ ;

MARUOKA, EM ;

CHOY, L ;

DERYNCK, R .

NATURE, 1995, 377 (6549) :548-552

[3] CLONING OF A TGF-BETA TYPE-I RECEPTOR THAT FORMS A HETEROMERIC COMPLEX WITH THE TGF-BETA TYPE-II RECEPTOR [J].

FRANZEN, P ;

TENDIJKE, P ;

ICHIJO, H ;

YAMASHITA, H ;

SCHULZ, P ;

HELDIN, CH ;

MIYAZONO, K .

CELL, 1993, 75 (04) :681-692

[4]

FYNAN TM, 1993, CRIT REV ONCOGENESIS, V4, P493

[5]

GARRIGUEANTAR L, 1995, CANCER RES, V55, P3982

[6] 'Long distance sequencer' method; A novel strategy for large DNA sequencing projects [J].

Hagiwara, K ;

Harris, CC .

NUCLEIC ACIDS RESEARCH, 1996, 24 (12) :2460-2461

[7] THE PROTEIN-KINASE FAMILY - CONSERVED FEATURES AND DEDUCED PHYLOGENY OF THE CATALYTIC DOMAINS [J].

HANKS, SK ;

QUINN, AM ;

HUNTER, T .

SCIENCE, 1988, 241 (4861) :42-52

[8]

KRAINER AR, 1988, FRONTIERS MOL BIOL T, P131

[9] EXPRESSION CLONING OF THE TGF-BETA TYPE-II RECEPTOR, A FUNCTIONAL TRANSMEMBRANE SERINE THREONINE KINASE [J].

LIN, HY ;

WANG, XF ;

NGEATON, E ;

WEINBERG, RA ;

LODISH, HF .

CELL, 1992, 68 (04) :775-785

[10] MUTATIONS OF THE TRANSFORMING GROWTH-FACTOR-BETA TYPE-II RECEPTOR GENE AND GENOMIC INSTABILITY IN HEREDITARY NONPOLYPOSIS COLORECTAL-CANCER [J].

LU, SL ;

AKIYAMA, Y ;

NAGASAKI, H ;

SAITOH, K ;

YUASA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 216 (02) :452-457

← 1 2 →