Clinical significance of enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid

被引：58
作者：
Amo, Y
Ohkawa, T
Tatsuta, M
Hamada, Y
Fujimura, T
Katsuoka, K
Hashimoto, T
机构：
[1] Kitasato Univ, Sch Med, Dept Dermatol, Sagamihara, Kanagawa 2288555, Japan
[2] Kurume Univ, Sch Med, Dept Dermatol, Kurume, Fukuoka 8300011, Japan
来源：
JOURNAL OF DERMATOLOGICAL SCIENCE | 2001年 / 26卷 / 01期
关键词：
autoantibody; bullous pemphigoid; BP180; recombinant protein;
D O I：
10.1016/S0923-1811(00)00149-3
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
The NC16A domain of the 180-kDa bullous pemphigoid antigen (BP180) is the most immunogenic and, probably, pathogenic region in bullous pemphigoid (BP). In the present study, in order to determine whether serum level of circulating anti-BP180 autoantibodies is a valuable serum marker in BP, the immunoreactivity of sera against the NC16A domain of BP180 was measured using enzyme-linked immunosorbent assay (ELISA) in ten patients with BP. Serum levels of anti-BP180 autoantibodies correlated with the clinical course in BP patients, who received various therapeutic agents. The result suggests that this NC16A-ELISA is a useful method for evaluating the clinical course and efficacy of the therapy in patients with BP. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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收藏
页码：14 / 18
页数：5
相关论文
共 10 条
[1]   BULLOUS PEMPHIGOID - CLINICAL AND IMMUNOLOGICAL FOLLOW-UP AFTER SUCCESSFUL THERAPY [J].
AHMED, AR ;
MAIZE, JC ;
PROVOST, TT .
ARCHIVES OF DERMATOLOGY, 1977, 113 (08) :1043-1046
[2]   DEVELOPMENT OF AN ELISA TO DETECT ANTI-BP180 AUTOANTIBODIES IN BULLOUS PEMPHIGOID AND HERPES-GESTATIONIS [J].
GIUDICE, GJ ;
WILSKE, KC ;
ANHALT, GJ ;
FAIRLEY, JA ;
TAYLOR, AF ;
EMERY, DJ ;
HOFFMAN, RG ;
DIAZ, LA .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (06) :878-881
[3]  
GIUDICE GJ, 1993, J IMMUNOL, V151, P5724
[4]   A PASSIVE TRANSFER MODEL OF THE ORGAN-SPECIFIC AUTOIMMUNE-DISEASE, BULLOUS PEMPHIGOID, USING ANTIBODIES GENERATED AGAINST THE HEMIDESMOSOMAL ANTIGEN, BP180 [J].
LIU, Z ;
DIAZ, LA ;
TROY, JL ;
TAYLOR, AF ;
EMERY, DJ ;
FAIRLEY, JA ;
GIUDICE, GJ .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (05) :2480-2488
[5]   A critical role for neutrophil elastase in experimental bullous pemphigoid [J].
Liu, Z ;
Shapiro, SD ;
Zhou, XY ;
Twining, SS ;
Senior, RM ;
Giudice, GJ ;
Fairley, JA ;
Diaz, LA .
JOURNAL OF CLINICAL INVESTIGATION, 2000, 105 (01) :113-123
[6]   The majority of bullous pemphigoid and herpes gestations serum samples read with the NC16a domain of the 180-kDa bullous pemphigoid antigen [J].
Matsumura, K ;
Amagai, M ;
Nishikawa, T ;
Hashimoto, T .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1996, 288 (09) :507-509
[7]  
Nakatani C, 1998, BRIT J DERMATOL, V139, P365
[8]   Serum levels of autoantibodies to BP180 correlate with disease activity in patients with bullous pemphigoid [J].
Schmidt, E ;
Obe, K ;
Bröcker, EB ;
Zillikens, D .
ARCHIVES OF DERMATOLOGY, 2000, 136 (02) :174-178
[9]   A highly sensitive enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid [J].
Zillikens, D ;
Mascaro, JM ;
Rose, PA ;
Liu, Z ;
Ewing, SM ;
Caux, F ;
Hoffmann, RG ;
Diaz, LA ;
Giudice, GJ .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1997, 109 (05) :679-683
[10]   THE INTERLEUKIN-2 RECEPTOR IN LESIONS AND SERUM OF BULLOUS PEMPHIGOID [J].
ZILLIKENS, D ;
AMBACH, A ;
SCHUESSLER, M ;
DUMMER, R ;
HARTMANN, AA ;
BURG, G .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 1992, 284 (03) :141-145
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