The structural basis of blebbistatin inhibition and specificity for myosin II

被引：256

作者：

Allingham, JS ^{[1
]}

Smith, R ^{[1
]}

Rayment, I ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 04期

关键词：

D O I：

10.1038/nsmb908

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular motors play a central role in cytoskeletal-mediated cellular processes and thus present an excellent target for cellular control by pharmacological agents. Yet very few such compounds have been found. We report here the structure of blebbistatin, which inhibits specific myosin isoforms, bound to the motor domain of Dictyostelium discoideum myosin II. This reveals the structural basis for its specificity and provides insight into the development of new agents.

引用

收藏

页码：378 / 379

页数：2

相关论文

共 15 条

[1] A millennial myosin census [J].

Berg, JS ;

Powell, BC ;

Cheney, RE .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (04) :780-794

[2] A small-molecule inhibitor of skeletal muscle myosin II [J].

Cheung, A ;

Dantzig, JA ;

Hollingworth, S ;

Baylor, SM ;

Goldman, YE ;

Mitchison, TJ ;

Straight, AF .

NATURE CELL BIOLOGY, 2002, 4 (01) :83-88

[3] Three myosin V structures delineate essential features of chemo-mechanical transduction [J].

Coureux, PD ;

Sweeney, HL ;

Houdusse, A .

EMBO JOURNAL, 2004, 23 (23) :4527-4537

[4] Inhibition of pancreatic adenocarcinoma cellular invasiveness by blebbistatin: a novel myosin II inhibitor [J].

Duxbury, MS ;

Ashley, SW ;

Whang, EE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 313 (04) :992-997

[5] Adhesion-contractile balance in myocyte differentiation [J].

Griffin, MA ;

Sen, S ;

Sweeney, HL ;

Discher, DE .

JOURNAL OF CELL SCIENCE, 2004, 117 (24) :5855-5863

[6] BUTANEDIONE MONOXIME SUPPRESSES CONTRACTION AND ATPASE ACTIVITY OF RABBIT SKELETAL-MUSCLE [J].

HIGUCHI, H ;

TAKEMORI, S .

JOURNAL OF BIOCHEMISTRY, 1989, 105 (04) :638-643

[7] Biochemical and molecular characterization of diseases linked to motor proteins [J].

Hirokawa, N ;

Takemura, R .

TRENDS IN BIOCHEMICAL SCIENCES, 2003, 28 (10) :558-565

[8] Mechanism of blebbistatin inhibition of myosin II [J].

Kovács, M ;

Tóth, J ;

Hetényi, C ;

Málnási-Csizmadia, A ;

Sellers, JR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (34) :35557-35563

[9] Specificity of blebbistatin, an inhibitor of myosin II [J].

Limouze, J ;

Straight, AF ;

Mitchison, T ;

Sellers, JE .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 2004, 25 (4-5) :337-341

[10] Unconventional myosins in cell movement, membrane traffic, and signal transduction [J].

Mermall, V ;

Post, PL ;

Mooseker, MS .

SCIENCE, 1998, 279 (5350) :527-533