Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production

被引：21

作者：

Dong, Xia ^{[1
,2
]}

Wu, Weiju ^{[2
]}

Ma, Liang ^{[2
]}

Liu, Chengfei ^{[3
]}

Bhuckory, Mohajeet B. ^{[4
]}

Wang, Liping ^{[1
]}

Nandrot, Emeline F. ^{[5
]}

Xu, Heping ^{[4
]}

Li, Ke ^{[3
]}

Liu, Yizhi ^{[1
]}

Zhou, Wuding ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou, Guangdong, Peoples R China

[2] Kings Coll London, Guys Hosp, MRC, Ctr Transplantat, 5th Floor Tower Wing, London SE1 9RT, England

[3] Xi An Jiao Tong Univ, Affiliated Hosp 2, Sch Med, Core Res Lab, Xian, Shaanxi, Peoples R China

[4] Queens Univ Belfast, Ctr Expt Med, Belfast, Antrim, North Ireland

[5] UPMC Univ Paris 06, CNRS, INSERM,U968,UMR S968, Inst Vis,UMR 7210,Dept Therapeut, Paris, France

来源：

JOURNAL OF INNATE IMMUNITY | 2017年 / 9卷 / 06期

基金：

中国国家自然科学基金; 英国医学研究理事会;

关键词：

Collectin; 11; CL-K1; Opsonophagocytosis; Cytokine production; Retinal pigment epithelial cells; LECTIN COMPLEMENT PATHWAY; ALPHA-V-BETA-5; INTEGRIN; INNATE IMMUNITY; 3MC SYNDROME; 11; CL-11; BINDING; CL-K1; CL-L1; PNEUMONIAE; ACTIVATION;

D O I：

10.1159/000478042

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this paper, we report previously unknown roles for collectin-11 (CL-11, a soluble C-type lectin) in modulating the retinal pigment epithelial (RPE) cell functions of phagocytosis and cytokine production. We found that CL-11 and its carbohydrate ligand are expressed in both the murine and human neural retina; these resemble each other in terms of RPE and photoreceptor cells. Functional analysis of murine RPE cells showed that CL-11 facilitates the opsonophagocytosis of photoreceptor outer segments and apoptotic cells, and also upregulates IL-10 production. Mechanistic analysis revealed that calreticulin on the RPE cells is required for CL-11-mediated opsonophagocytosis whereas signal-regulatory protein a and mannosyl residues on the cells are involved in the CL-11-mediated upregulation of IL-10 production. This study is the first to demonstrate the role of CL-11 and the molecular mechanisms involved in modulating RPE cell phagocytosis and cytokine production. It provides a new insight into retinal health and disease and has implications for other phagocytic cells. (C) 2017 S. Karger AG, Basel.

引用

页码：529 / 545

页数：17

共 33 条

[1] A2E Induces IL-1β Production in Retinal Pigment Epithelial Cells via the NLRP3 Inflammasome [J].