MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis

被引:222
作者
Buschow, Sonja I. [1 ,2 ]
van Balkom, Bas W. M. [3 ,4 ]
Aalberts, Marian [1 ,2 ]
Heck, Albert J. R. [3 ,4 ]
Wauben, Marca [1 ,2 ]
Stoorvogel, Willem [1 ,2 ]
机构
[1] Univ Utrecht, Dept Biochem & Cell Biol, Fac Vet Med, NL-3508 TD Utrecht, Netherlands
[2] Univ Utrecht, Inst Biomembranes, NL-3508 TD Utrecht, Netherlands
[3] Univ Utrecht, Bijvoet Ctr Biomol Res, Dept Biomol Mass Spectrometry, NL-3508 TD Utrecht, Netherlands
[4] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3508 TD Utrecht, Netherlands
关键词
exosomes; MHC class II; sorting; heat shock proteins; ASSISTED-LASER-DESORPTION/IONIZATION; TANDEM MASS-SPECTRA; DENDRITIC CELLS; MULTIVESICULAR ENDOSOMES; PROTEOMIC ANALYSIS; RETICULOCYTE MATURATION; TRANSFERRIN RECEPTOR; INTERNAL VESICLES; IMMUNE-RESPONSES; PLASMA-MEMBRANE;
D O I
10.1038/icb.2010.64
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Professional antigen-presenting cells secrete major histocompatibility complex class II (MHC II) carrying exosomes with unclear physiological function(s). Exosomes are first generated as the intraluminal vesicles (ILVs) of a specific type of multivesicular body, and are then secreted by fusion of this compartment with the plasma membrane. We have previously shown that in contrast to the sorting of MHC II at lysosomally targeted multivesicular bodies, sorting of MHC II into exosomes does not rely on MHC II ubiquitination. In search for proteins that drive the incorporation of MHC II into exosomes or functionally discriminate exosomal from plasma membrane MHC II, we first analyzed the total proteome of highly purified B cell-derived exosomes using sensitive and accurate mass spectrometry (MS), and identified 539 proteins, including known and not previously identified constituents. Using quantitative MS, we then identified a small subset of proteins that were specifically co-immunoprecipitated with MHC II from detergent-solubilized exosomes. These include HSC71, HSP90, 14-3-3 epsilon, CD20 and pyruvate kinase type M2 (PKM2), and we speculate on the functionality of their interaction with exosomal MHC II. Immunology and Cell Biology (2010) 88, 851-856; doi:10.1038/icb.2010.64; published online 11 May 2010
引用
收藏
页码:851 / 856
页数:6
相关论文
共 64 条
[1]   Proteomic analysis of the tetraspanin web using LC-ESI-MS/MS and MALDI-FTICR-MS [J].
André, M ;
Le Caer, JP ;
Greco, C ;
Planchon, S ;
El Nemer, W ;
Boucheix, C ;
Rubinstein, E ;
Chamot-Rooke, J ;
Le Naour, F .
PROTEOMICS, 2006, 6 (05) :1437-1449
[2]   A protein's final ESCRT [J].
Babst, M .
TRAFFIC, 2005, 6 (01) :2-9
[3]   Ontologizer 2.0 - a multifunctional tool for GO term enrichment analysis and data exploration [J].
Bauer, Sebastian ;
Grossmann, Steffen ;
Vingron, Martin ;
Robinson, Peter N. .
BIOINFORMATICS, 2008, 24 (14) :1650-1651
[4]   Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release [J].
Broquet, AH ;
Thomas, G ;
Masliah, J ;
Trugnan, G ;
Bachelet, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (24) :21601-21606
[5]   Investigation of doxorubicin resistance in MCF-7 breast cancer cells using shot-gun comparative proteomics with proteolytic 18O labeling [J].
Brown, KJ ;
Fenselau, C .
JOURNAL OF PROTEOME RESEARCH, 2004, 3 (03) :455-462
[6]   MHC II in Dendritic Cells is Targeted to Lysosomes or T Cell-Induced Exosomes Via Distinct Multivesicular Body Pathways [J].
Buschow, Sonja I. ;
Nolte-'t Hoen, Esther N. M. ;
van Niel, Guillaume ;
Pols, Maaike S. ;
ten Broeke, Toine ;
Lauwen, Marjolein ;
Ossendorp, Ferry ;
Melief, Cornelis J. M. ;
Raposo, Graca ;
Wubbolts, Richard ;
Wauben, Marca H. M. ;
Stoorvogel, Willem .
TRAFFIC, 2009, 10 (10) :1528-1542
[7]   The potential of exosomes in immunotherapy of cancer [J].
Chaput, N ;
Taïeb, J ;
Schartz, N ;
Flament, C ;
Novault, S ;
André, F ;
Zitvogel, L .
BLOOD CELLS MOLECULES AND DISEASES, 2005, 35 (02) :111-115
[8]   The tetraspanin CD81 is necessary for partitioning of coligated CD19/CD21-B cell antigen receptor complexes into signaling-active lipid rafts [J].
Cherukuri, A ;
Shoham, T ;
Sohn, HW ;
Levy, S ;
Brooks, S ;
Carter, R ;
Pierce, SK .
JOURNAL OF IMMUNOLOGY, 2004, 172 (01) :370-380
[9]   Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes [J].
Chi, An ;
Valencia, Julio C. ;
Hu, Zhang-Zhi ;
Watabe, Hidenori ;
Yamaguchi, Hiroshi ;
Mangini, Nancy J. ;
Huang, Hongzhan ;
Canfield, Victor A. ;
Cheng, Keith C. ;
Yang, Feng ;
Abe, Riichiro ;
Yamagishi, Shoichi ;
Shabanowitz, Jeffrey ;
Hearing, Vincent J. ;
Wu, Cathy ;
Appella, Ettore ;
Hunt, Donald F. .
JOURNAL OF PROTEOME RESEARCH, 2006, 5 (11) :3135-3144
[10]   CD81 associates with 14-3-3 in a redox-regulated palmitoylation-dependent manner [J].
Clark, KL ;
Oelke, A ;
Johnson, ME ;
Eilert, KD ;
Simpson, PC ;
Todd, SC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (19) :19401-19406