共 64 条
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis
被引:222
作者:
Buschow, Sonja I.
[1
,2
]
van Balkom, Bas W. M.
[3
,4
]
Aalberts, Marian
[1
,2
]
Heck, Albert J. R.
[3
,4
]
Wauben, Marca
[1
,2
]
Stoorvogel, Willem
[1
,2
]
机构:
[1] Univ Utrecht, Dept Biochem & Cell Biol, Fac Vet Med, NL-3508 TD Utrecht, Netherlands
[2] Univ Utrecht, Inst Biomembranes, NL-3508 TD Utrecht, Netherlands
[3] Univ Utrecht, Bijvoet Ctr Biomol Res, Dept Biomol Mass Spectrometry, NL-3508 TD Utrecht, Netherlands
[4] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3508 TD Utrecht, Netherlands
关键词:
exosomes;
MHC class II;
sorting;
heat shock proteins;
ASSISTED-LASER-DESORPTION/IONIZATION;
TANDEM MASS-SPECTRA;
DENDRITIC CELLS;
MULTIVESICULAR ENDOSOMES;
PROTEOMIC ANALYSIS;
RETICULOCYTE MATURATION;
TRANSFERRIN RECEPTOR;
INTERNAL VESICLES;
IMMUNE-RESPONSES;
PLASMA-MEMBRANE;
D O I:
10.1038/icb.2010.64
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Professional antigen-presenting cells secrete major histocompatibility complex class II (MHC II) carrying exosomes with unclear physiological function(s). Exosomes are first generated as the intraluminal vesicles (ILVs) of a specific type of multivesicular body, and are then secreted by fusion of this compartment with the plasma membrane. We have previously shown that in contrast to the sorting of MHC II at lysosomally targeted multivesicular bodies, sorting of MHC II into exosomes does not rely on MHC II ubiquitination. In search for proteins that drive the incorporation of MHC II into exosomes or functionally discriminate exosomal from plasma membrane MHC II, we first analyzed the total proteome of highly purified B cell-derived exosomes using sensitive and accurate mass spectrometry (MS), and identified 539 proteins, including known and not previously identified constituents. Using quantitative MS, we then identified a small subset of proteins that were specifically co-immunoprecipitated with MHC II from detergent-solubilized exosomes. These include HSC71, HSP90, 14-3-3 epsilon, CD20 and pyruvate kinase type M2 (PKM2), and we speculate on the functionality of their interaction with exosomal MHC II. Immunology and Cell Biology (2010) 88, 851-856; doi:10.1038/icb.2010.64; published online 11 May 2010
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页码:851 / 856
页数:6
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