Oxidation and flow-injection amperometric determination of 5-hydroxytryptophan at an electrode modified by electrochemically assisted deposition of a sol-gel film with templated nanoscale pores

被引：19

作者：

Ranganathan, David ^{[1
,2
]}

Zamponi, Silvia ^{[2
]}

Berrettoni, Mario ^{[3
,4
]}

Mehdi, B. Layla ^{[1
]}

Cox, James A. ^{[1
]}

机构：

[1] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA

[2] Univ Camerino, Dept Chem Sci, I-62032 Camerino, Italy

[3] Univ Bologna, Dept Phys & Inorgan Chem, I-40136 Bologna, Italy

[4] INSTM Bologna, Unita Ric, I-40136 Bologna, Italy

来源：

TALANTA | 2010年 / 82卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Sol-gel films; Electrochemically assisted deposition; 5-Hydroxytryptophan; Voltammetry; Flow-injection analysis; MODIFIED GLASSY-CARBON; COBALT HEXACYANOFERRATE; ELECTROCATALYTIC OXIDATION; SILICA MICROSPHERES; HYDROLYSIS; DENDRIMERS; SURFACES; ANILINE;

D O I：

10.1016/j.talanta.2010.06.025

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The oxidation of 5-hydroxytryptophan (5-HTPP) yielded a passivating polymeric film at an indium tin oxide (ITO) electrode. Coating ITO with a nanoscale sol-gel film with a mesoporous structure was shown to change the pathway of the chemical reaction coupled to the electron transfer. The sol-gel film was deposited by an electrochemically assisted process, and the mesoporosity was imparted by including generation-4 poly(amidoamine) dendrimer in the precursor solution. The dendrimer was removed subsequently with an atmospheric oxygen plasma. This electrode remained active during cyclic voltammetry and controlled potential electrolysis of 5-HTPP, which was attributed to dimer, rather than polymer, formation from the oxidation product. Mass spectrometry confirmed this hypothesis. The anodic current was limited by the electron-transfer kinetics. Modification of the so-gel film by inclusion of cobalt hexacyanoferrate, which catalyzes the oxidation, resulted in a diffusion-limited current. Determination of 5-HTPP by flow-injection amperometry had a detection limit of 17 nM. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1149 / 1155

页数：7

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