Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42

被引:647
|
作者
Chen, Xiao Hua
Koumoutsi, Alexandra
Scholz, Romy
Eisenreich, Andreas
Schneider, Kathrin
Heinemeyer, Isabelle
Morgenstern, Burkhard
Voss, Bjoern
Hess, Wolfgang R.
Reva, Oleg
Junge, Helmut
Voigt, Birgit
Jungblut, Peter R.
Vater, Joachim
Suessmuth, Roderich
Liesegang, Heiko
Strittmatter, Axel
Gottschalk, Gerhard
Borriss, Rainer
机构
[1] Univ Gottingen, Inst Mikrobiol & Genet, Genome Anal Lab, D-37077 Gottingen, Germany
[2] Humboldt Univ, Inst Biol, D-10115 Berlin, Germany
[3] Tech Univ Berlin, Inst Biol Chem, D-10587 Berlin, Germany
[4] Univ Gottingen, Inst Mikrobiol & Genet, D-37073 Gottingen, Germany
[5] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany
[6] Univ Pretoria, Dept Biochem, Bioinformat & Computat Biol Unit, ZA-0002 Pretoria, South Africa
[7] Abitep GmbH, Glienicker Weg 185, D-12489 Berlin, Germany
[8] Univ Greifswald, Inst Mikrobiol & Mol Biol, D-17487 Greifswald, Germany
[9] MPI Infekt Biol, D-10117 Berlin, Germany
关键词
D O I
10.1038/nbt1325
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. Its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, which occur ubiquitously in the closely related Bacillus subtilis 168 genome. The B. amyloliquefaciens FZB42 genome reveals an unexpected potential to produce secondary metabolites, including the polyketides bacillaene and difficidin. More than 8.5% of the genome is devoted to synthesizing antibiotics and siderophores by pathways not involving ribosomes. Besides five gene clusters, known from B. subtilis to mediate nonribosomal synthesis of secondary metabolites, we identified four giant gene clusters absent in B. subtilis 168. The pks2 gene cluster encodes the components to synthesize the macrolactin core skeleton.
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收藏
页码:1007 / 1014
页数:8
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