In Situ Monitoring of Photodynamic Inactivation of the Membrane Functions of Bacteria Using Electrochemical Sensors

被引：8
作者：
Kato, Hisato [1 ]
Komagoe, Keiko [1 ]
Inoue, Tsuyoshi [1 ]
Katsu, Takashi [1 ]
机构：
[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Kita Ku, Okayama 7008530, Japan
来源：
ANALYTICAL SCIENCES | 2010年 / 26卷 / 10期
基金：
日本学术振兴会;
关键词：
OXYGEN-ELECTRODE; GRAMICIDIN-S;
D O I：
10.2116/analsci.26.1019
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The photodynamic inactivation of the membrane functions of bacteria was analyzed in still, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.
引用
收藏
页码：1019 / 1021
页数：3
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