Mechanical stress rapidly induces E-resveratrol and E-piceatannol biosynthesis in grape canes stored as a freshly-pruned byproduct

被引：44

作者：

Billet, Kevin ^{[1
]}

Houille, Benjamin ^{[1
]}

Besseau, Sebastien ^{[1
]}

Melin, Celine ^{[1
]}

Oudin, Audrey ^{[1
]}

Papon, Nicolas ^{[2
]}

Courdavault, Vincent ^{[1
]}

Clastre, Marc ^{[1
]}

Giglioli-Guivarc'h, Nathalie ^{[1
]}

Lanoue, Arnaud ^{[1
]}

机构：

[1] Univ Francois Rabelais Tours, EA Biomol & Biotechnol Vegetales 2106, UFR Sci Pharmaceut, 31 Av Monge, F-37200 Tours, France

[2] Univ Angers, Grp Etud Interact Hote Pathogene, Angers, France

来源：

FOOD CHEMISTRY | 2018年 / 240卷

关键词：

E-resveratrol; E-piceatannol; Grape canes; Byproduct; Stress induction; Stilbenoid biosynthesis; Mechanical wounding; PLASMOPARA-VITICOLA; STILBENE SYNTHASE; POWDERY MILDEW; VINIFERA-L; UV-C; SCOTS PINE; VITIS; INDUCTION; DEFENSE; PLANTS;

D O I：

10.1016/j.foodchem.2017.07.105

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Grape canes represent a promising source of bioactive phytochemicals. However the stabilization of the raw material after pruning remains challenging. We recently reported the induction of stilbenoid metabolism after winter pruning including a strong accumulation of E-resveratrol and E-piceatannol during the first six weeks of storage. In the present study, the effect of mechanical wounding on freshly-pruned canes was tested to increase the induction of stilbenoid metabolism. Cutting the grape canes in short segments immediately after pruning triggered a transient expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes, followed by a rapid accumulation of E-resveratrol and E-piceatannol. The degree of stilbenoid induction was related to the intensity of mechanical wounding. Data suggest that a global defense response is triggered involving jasmonate signaling, PR proteins and stilbenoid metabolism. Mechanical wounding of freshly-pruned canes drastically shortens the time required to reach maximal stilbenoid accumulation from 6 to 2 weeks.

引用

页码：1022 / 1027

页数：6

共 35 条

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