Proteomic Profiling of Proteins Associated With Lymph Node Metastasis in Colorectal Cancer

被引:49
作者
Ma, Yiming [2 ,3 ]
Zhao, Mei [2 ,3 ]
Zhong, Jialing [2 ,3 ]
Shi, Lan [2 ,3 ]
Luo, Qing [2 ,3 ]
Liu, Jian [2 ,3 ]
Wang, Jia [2 ,3 ]
Yuan, Xinghua [1 ,2 ]
Huang, Changzhi [2 ,3 ]
机构
[1] Peking Union Med Coll, Canc Hosp, Dept Abdomen Surg, Beijing 100021, Peoples R China
[2] Chinese Acad Med Sci, Beijing 100021, Peoples R China
[3] Peking Union Med Coll, Canc Inst Hosp, Key Lab Mol Oncol, Beijing 100021, Peoples R China
关键词
PROTEOMICS; LYMPH NODE METASTASIS; COLORECTAL CANCER; UCH-L1; SQUAMOUS-CELL CARCINOMA; CATHEPSIN-D EXPRESSION; C-TERMINAL HYDROLASE; BREAST-CANCER; PROGNOSTIC-FACTOR; COLON-CANCER; DIFFERENTIAL EXPRESSION; SCREENING SIGMOIDOSCOPY; GEL-ELECTROPHORESIS; PGP9.5;
D O I
10.1002/jcb.22726
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lymph node metastasis (LNM) is associated with poor prognosis in colorectal cancer (CRC). The presence or absence of lymph node metastases is a strong independent prognostic factor for CRC survival. Investigation of proteins associated with the process of lymph node metastasis (LNM) is crucial for understanding of the molecular mechanisms underlying the LNM process and for predicting the CRC prognosis. In the present study, proteins from CRC tissues and adjacent normal mucosa (NMC) were examined using two-dimensional gel electrophoresis coupled with MALDI-TOF-MS. The expression levels of Ferritin Heavy Chain (FHC) were decreased in LNM CRC as compared to those in non-LNM CRC, while the expression of Cathepsin D and Ubiquitin C-terminal hydrolase-L1 (UCH-L1) were increased in LNM CRC. The results were confirmed by Western blotting and immunohistochemical staining. Furthermore, in vitro cell invasion assay showed that the overexpression of UCH-L1 through gene transfection increased the invasive ability of HCT8 cells, suggesting that UCH-L1 is not only a biomarker for LNM in CRC, but also a functional protein that may play a significant role in cell migration. The proteins identified in the present study should further our understanding of the LNM process of CRC and may become useful markers for diagnosis and targets for therapeutic interventions. J. Cell. Biochem. 110: 1512-1519, 2010. (C) 2010 Wiley-Liss, Inc.
引用
收藏
页码:1512 / 1519
页数:8
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