Effects of low iron conditions on the repair of DNA lesions induced by Cumene hydroperoxide in Escherichia coli cells

被引：4
作者：
Asad, LMBO
Medeiros, DC
Felzenszwalb, I
Leitao, AC
Asad, NR [1 ]
机构：
[1] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Biofis & Biometria, BR-20551030 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Radiobiol Mol, BR-21949900 Rio De Janeiro, Brazil
来源：
MUTATION RESEARCH-DNA REPAIR | 2001年 / 485卷 / 04期
关键词：
Escherichia coli; cumene hydroperoxide; iron chelator; DNA lesions; mutagenesis;
D O I：
10.1016/S0921-8777(01)00073-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In the present study, we evaluated the sensitivity of different Escherichia coli strains to Cumene hydroperoxide (CHP) treatment under distinct conditions of Fe2+ availability. Our results showed that the pretreatment with an iron chelator (dipyridyl) protects all the tested strains against CHP toxic effects, but it was not sufficient to abolish the CHP induced mutagenesis. On the other hand, simultaneous pretreatment with both dipyridyl and neocuproine (copper chelator) leads to a complete protection against CHB mutagenic effects. Our data suggest the participation of copper ion in the CHP mutagenesis induced in E. coli. (C) 2001 Published by Elsevier Science B.V.
引用
收藏
页码：339 / 344
页数：6
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