Use of ethanolamine for sample stacking in capillary electrophoresis

被引：3
|
作者：
Louwagie, M
Rabilloud, T
Garin, J
机构：
[1] CEA, Dept Biol Mol & Struct, Lab Chim Prot, F-38054 Grenoble, France
[2] CEA, Dept Biol Mol & Struct, Lab Bioenerget Cellulaire & Pathol, F-38054 Grenoble, France
来源：
ELECTROPHORESIS | 1998年 / 19卷 / 14期
关键词：
capillary electrophoresis; sample stacking; microsequence analysis;
D O I：
10.1002/elps.1150191414
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Capillary zone electrophoresis (CZE) in the presence of ethanolamine was used in a micropreparative mode. Sample volumes up to 1 mu L could be loaded onto a 100 mu m diameter capillary without loss in resolution. Coupled to narrow-bore reversed-phase high-performance liquid chromatography, ethanolamine-CZE allowed the collection of sufficient amounts of pure peptidic material to perform amino acid sequence analysis.
引用
收藏
页码：2440 / 2444
页数：5
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