Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster

被引：0

作者：

Boyko, K. M. ^{[1
,2
]}

Nikolaeva, A. Yu. ^{[1
,2
]}

Kachalova, G. S. ^{[2
]}

Bonchuk, A. N. ^{[3
]}

Dorovatovskii, P. V. ^{[2
]}

Popov, V. O. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia

[2] Natl Res Ctr, Kurchatov Inst, Moscow 123098, Russia

[3] Russian Acad Sci, Inst Gene Biol, Moscow 119334, Russia

来源：

CRYSTALLOGRAPHY REPORTS | 2017年 / 62卷 / 06期

关键词：

CRYSTAL-STRUCTURE; INTERFACE; GENOME;

D O I：

10.1134/S1063774517060062

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The Drosophila genome has several dozens of transcription factors (TTK group) containing D'D cent D' domains assembled into octamers. The LOLA protein belongs to this family. The purification, crystallization, and preliminary X-ray diffraction and small-angle X-ray scattering (SAXS) studies of the BTB domain of this protein are reported. The crystallization conditions were found by the vapor-diffusion technique. A very low diffraction resolution (8.7 resolution) of the crystals was insufficient for the determination of the threedimensional structure of the BTB domain. The SAXS study demonstrated that the BTB domain of the LOLA protein exists as an octamer in solution.

引用

页码：912 / 915

页数：4

共 21 条

[11] PRIMUS:: a Windows PC-based system for small-angle scattering data analysis [J].

Konarev, PV ;

Volkov, VV ;

Sokolova, AV ;

Koch, MHJ ;

Svergun, DI .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2003, 36 :1277-1282

[12] Enhancer-promoter communication is regulated by insulator pairing in a Drosophila model bigenic locus [J].

Maksimenko, Oksana ;

Golovnin, Anton ;

Georgiev, Pavel .

MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (17) :5469-5477

[13] Mechanisms and proteins involved in long-distance interactions [J].

Maksimenko, Oksana ;

Georgiev, Pavel .

FRONTIERS IN GENETICS, 2014, 5

[14] Spatial partitioning of the regulatory landscape of the X-inactivation centre [J].

Nora, Elphege P. ;

Lajoie, Bryan R. ;

Schulz, Edda G. ;

Giorgetti, Luca ;

Okamoto, Ikuhiro ;

Servant, Nicolas ;

Piolot, Tristan ;

van Berkum, Nynke L. ;

Meisig, Johannes ;

Sedat, John ;

Gribnau, Joost ;

Barillot, Emmanuel ;

Bluethgen, Nils ;

Dekker, Job ;

Heard, Edith .

NATURE, 2012, 485 (7398) :381-385

[15] ATSAS 2.1 -: towards automated and web-supported small-angle scattering data analysis [J].

Petoukhov, Maxim V. ;

Konarev, Peter V. ;

Kikhney, Alexey G. ;

Svergun, Dmitri I. .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2007, 40 :S223-S228

[16] The BTB/POZ domain of the regulatory proteins Bric a brac 1 (BAB1) and Bric a brac 2 (BAB2) interacts with the novel Drosophila TAFII factor BIP2/dTAFII155 [J].

Pointud, JC ;

Larsson, J ;

Dastague, B ;

Couderc, JL .

DEVELOPMENTAL BIOLOGY, 2001, 237 (02) :368-380

[17]

Silva D., 2016, PLOS ONE, V11, P1

[18] A beta-sheet interaction interface directs the tetramerisation of the Miz-1 POZ domain [J].

Stead, Mark A. ;

Trinh, Chi H. ;

Garnett, James A. ;

Carr, Stephen B. ;

Baron, Andrew J. ;

Edwards, Thomas A. ;

Wright, Stephanie C. .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 373 (04) :820-826

[19] Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator [J].

Stogios, Peter J. ;

Chen, Lu ;

Prive, Gilbert G. .

PROTEIN SCIENCE, 2007, 16 (02) :336-342

[20] Insights into Strand Exchange in BTB Domain Dimers from the Crystal Structures of FAZF and Miz1 [J].

Stogios, Peter J. ;

Cuesta-Seijo, Jose Antonio ;

Chen, Lu ;

Pomroy, Neil C. ;

Prive, Gilbert G. .

JOURNAL OF MOLECULAR BIOLOGY, 2010, 400 (05) :983-997

← 1 2 3 →