Non-Invasive Photothermal Strain Imaging of Non-Alcoholic Fatty Liver Disease in Live Animals

被引:5
作者
Choi, Changhoon [1 ]
Choi, Wonseok [1 ]
Kim, Jeesu [1 ,2 ]
Kim, Chulhong [3 ,4 ]
机构
[1] Pohang Univ Sci & Technol, Med Device Innovat Ctr, Dept Convergence IT Engn, Pohang 37673, South Korea
[2] Pusan Natl Univ, Coll Nanosci & Nanotechnol, Dept Cognomechatron Engn, Busan 46241, South Korea
[3] Pohang Univ Sci & Technol, Dept Elect Engn Convergence IT Engn & Mech Engn, Pohang 37673, South Korea
[4] Pohang Univ Sci & Technol, Med Device Innovat Ctr, Pohang 37673, South Korea
基金
新加坡国家研究基金会;
关键词
Silicides; Platinum alloys; Imaging; Strain; Heating systems; Fats; Laser beams; Photothermal strain imaging; preclinical research; NAFLD; tissue characterization; ACOUSTIC STRUCTURE QUANTIFICATION; HEPATIC STEATOSIS; ULTRASOUND; US; CT; VARIABILITY; FIBROSIS; BIOPSY; MODEL;
D O I
10.1109/TMI.2021.3081097
中图分类号
TP39 [计算机的应用];
学科分类号
081203 ; 0835 ;
摘要
The prevalence of non-alcoholic fatty liver diseases (NAFLD) has increased steadily over the past decade. Thus, diagnosing NAFLD at the earliest stage, which is a reversible condition, has become increasingly important. Here, photothermal strain imaging (pTSI) is presented as a novel non-invasive tool for NAFLD diagnosis. The pTSI uses ultrasound to detect the difference in thermal strain between fat and water during a light-induced temperature rise, which is directly related to the pathological evidence of NAFLD. To demonstrate its feasibility, fat accumulation in in vivo rat livers is monitored non-invasively using pTSI, based on clinical ultrasound B-mode images. A total of 21 male Wistar rats of 3 weeks of age were prepared. Of these, 18 rats received methionine-choline deficient diet for 1 to 6 weeks (n = 3 per week) to induce NAFLD, whereas 3 rats received normal diet as controls (n = 3). Livers were heated by a lipid-sensitive continuous-wave laser, and strain was measured. Quantitative results from the pTSI were compared with histological analysis results using Oil-Red-O (ORO). The receiver operating characteristic curve of in vivo pTSI results for detecting moderate steatosis (ORO-stained area >= 33%) was constructed based on strain change rate measured in the liver region. The sensitivity and specificity of pTSI were 90% and 82%, respectively, and the area-under-the-curve was measured as 0.85 +/- 0.03 (95% confidence interval). The pTSI results tested in the rodent NAFLD model showed great potential for pTSI to be used as a new diagnostic tool for NAFLD in the future.
引用
收藏
页码:2487 / 2495
页数:9
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