Impact of Spaceflight and Artificial Gravity on the Mouse Retina: Biochemical and Proteomic Analysis

被引：54

作者：

Mao, Xiao W. ^{[1
]}

Byrum, Stephanie ^{[2
,3
]}

Nishiyama, Nina C. ^{[1
]}

Pecaut, Michael J. ^{[1
]}

Sridharan, Vijayalakshmi ^{[4
]}

Boerma, Marjan ^{[4
]}

Tackett, Alan J. ^{[2
,3
]}

Shiba, Dai ^{[5
]}

Shirakawa, Masaki ^{[5
]}

Takahashi, Satoru ^{[6
]}

Delp, Michael D. ^{[7
]}

机构：

[1] Loma Linda Univ, Sch Med & Med Ctr, Div Biomed Engn Sci BMES, Dept Basic Sci, Loma Linda, CA 92350 USA

[2] Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA

[3] Arkansas Childrens Res Inst, Little Rock, AR 72202 USA

[4] Univ Arkansas Med Sci, Dept Pharmaceut Sci, Div Radiat Hlth, Little Rock, AR 72205 USA

[5] JAXA, JEM Utilizat Ctr, Human Spaceflight Technol Directorate, Tsukuba, Ibaraki 3058505, Japan

[6] Univ Tsukuba, Dept Anat & Embryol, Tsukuba, Ibaraki 3058575, Japan

[7] Florida State Univ, Dept Nutr Food & Exercise Sci, Tallahassee, FL 32306 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 09期

基金：

美国国家卫生研究院;

关键词：

spaceflight; ocular tissue; microgravity; artificial gravity; apoptosis; proteomics; oxidative stress; WILLIAMS-SYNDROME; PROTEIN; MECP2; CLIP-115; BRAIN;

D O I：

10.3390/ijms19092546

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Astronauts are reported to have experienced some impairment in visual acuity during their mission on the International Space Station (ISS) and after they returned to Earth. There is emerging evidence that changes in vision may involve alterations in ocular structure and function. To investigate possible mechanisms, changes in protein expression profiles and oxidative stress-associated apoptosis were examined in mouse ocular tissue after spaceflight. Nine-week-old male C57BL/6 mice (n = 12) were launched from the Kennedy Space Center on a SpaceX rocket to the ISS for a 35-day mission. The animals were housed in the mouse Habitat Cage Unit (HCU) in the Japan Aerospace Exploration Agency (JAXA) "Kibo" facility on the ISS. The flight mice lived either under an ambient microgravity condition (mu g) or in a centrifugal habitat unit that produced 1 g artificial gravity (mu g + 1 g). Habitat control (HC) and vivarium control mice lived on Earth in HCUs or normal vivarium cages, respectively. Quantitative assessment of ocular tissue demonstrated that the mu g group induced significant apoptosis in the retina vascular endothelial cells compared to all other groups (p < 0.05) that was 64% greater than that in the HC group. Proteomic analysis showed that many key pathways responsible for cell death, cell repair, inflammation, and metabolic stress were significantly altered in mu g mice compared to HC animals. Additionally, there were more significant changes in regulated protein expression in the mu g group relative to that in the mu g + 1 g group. These data provide evidence that spaceflight induces retinal apoptosis of vascular endothelial cells and changes in retinal protein expression related to cellular structure, immune response and metabolic function, and that artificial gravity (AG) provides some protection against these changes. These retinal cellular responses may affect blood-retinal barrier (BRB) integrity, visual acuity, and impact the potential risk of developing late retinal degeneration.

引用

页数：15

共 25 条

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