Determination of [S,S′]-ethylenediamine disuccinic acid (EDDS) by high performance liquid chromatography after derivatization with FMOC

被引：28

作者：

Tandy, S

Schulin, R

Suter, MJF

Nowack, B

机构：

[1] Swiss Fed Inst Technol, Inst Terr Ecol, CH-8952 Schlieren, Switzerland

[2] Swiss Fed Inst Environm Sci & Technol, CH-8600 Dubendorf, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1077卷 / 01期

关键词：

ethylenediamine disuccinic acid; HPLC; fluorescence detection; EDDS; mass spectrometric identification;

D O I：

10.1016/j.chroma.2005.04.064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The paper describes a new HPLC method for the determination of ethylenediamine disuccinic acid (EDDS). EDDS is derivatized with FMOC reagent followed by HPLC separation on a reversed-phase column. The eluents consist of phosphate buffer at pH 6.8 and acetonitrile. Separation was carried out using gradient elution. The FMOC-EDDS derivative is detected with a fluorescence detector with an excitation wavelength of 265 nm and an emission wavelength of 313 nm. The detection limit is 0.01 mu M. The method is applicable to the determination of the compound in water, soil solution and plant material at trace levels. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：37 / 43

页数：7

共 23 条

[1] Determination of strong binding chelators and their metal complexes by anion-exchange chromatography and inductively coupled plasma mass spectrometry [J].