CXCR4 promotes metastases to the lung of osteosarcoma xenografts in mice

被引：0

作者：

Chen, Shunyou ^{[1
,2
]}

Zhang, Tao ^{[2
]}

Pan, Yuancheng ^{[2
]}

Lin, Ran ^{[2
]}

Chen, Dongdong ^{[2
]}

Weng, Yan ^{[2
]}

Yu, Bin ^{[1
]}

Zhang, Yiyuan ^{[2
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Dept Orthoped & Traumatol, 1813 North Guangzhou Rd, Guangzhou 510515, Guangdong, Peoples R China

[2] Xiamen Univ, Fuzhou Hosp 2, Dept Orthoped, Fuzhou, Fujian, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2018年 / 11卷 / 09期

关键词：

CXCR4; osteosarcoma; pulmonary metastasis; VEGF; TIMP-1; MESENCHYMAL STEM-CELLS; BREAST-CANCER; UP-REGULATION; PATHWAYS; PHENOTYPE; CARCINOMA; CYTOKINES; INVASION; PROGRESS; BIOLOGY;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objectives: To investigate the effect of CXCR4 on the metastasis of osteosarcoma cells. Methods: The in vitro invasion and migration of osteosarcoma MG-63 cells was assessed by transwell assay. Bioluminescence was applied to evaluate the metastasis of osteosarcoma cells in a mouse xenograft model. Changes in the expression of the metastasis-related cytokines vascular endothelial growth factor (VEGF), matrix metallopeptidase 9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were assessed by western blotting. Results: A significantly (p<0.05) higher invasion ability was observed in MG-63 cells with CXCR4 overexpression by transwell assay. A similar size of orthotopic osteosarcoma but more pulmonary tumors were found in mice injected with CXCR4-overexpressing MG63 cells than the control. Moreover, the expressions of VEGF and MMP-9 in the groups with CXCR4 overexpression were significantly (p<0.05) higher than those in the control; however, the opposite results were found for TIMP-1 in cultured MG-63 cells and in mouse orthotopic osteosarcoma and pulmonary tumors. Conclusions: CXCR4 up-regulates the expression of VEGF and MMP-9 but down-regulates TIMP-1, thereby promoting lung metastases of human osteosarcoma cells. Our findings suggest that CXCR4 is a potential target for the treatment of human osteosarcoma.

引用

页码：9441 / 9447

页数：7

共 25 条

[21] Upregulation of RICTOR gene transcription by the proinflammatory cytokines through NF-κB pathway contributes to the metastasis of renal cell carcinoma [J].