Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1

被引：35

作者：

Fernandez-Martinez, Ana B. ^{[1
]}

Bajo, Ana M. ^{[1
]}

Isabel Arenas, M. ^{[2
]}

Sanchez-Chapado, Manuel ^{[3
,4
]}

Prieto, Juan C. ^{[1
]}

Carmena, Maria J.

机构：

[1] Univ Alcala de Henares, Dept Biochem & Mol Biol, Alcala De Henares 28871, Spain

[2] Univ Alcala de Henares, Dept Cell Biol & Genet, Alcala De Henares 28871, Spain

[3] Univ Alcala de Henares, Dept Surg, Alcala De Henares 28871, Spain

[4] Principe Asturias Hosp, Dept Urol, Alcala De Henares 28871, Spain

来源：

CANCER LETTERS | 2010年 / 299卷 / 01期

关键词：

VIP; RWPE-1; cells; EMT; Tumorigenesis; Prostate cancer; PROTEIN-COUPLED RECEPTORS; MESENCHYMAL TRANSITION; NEUROENDOCRINE DIFFERENTIATION; ANTAGONISTIC ACTION; ANDROGEN RECEPTOR; HORMONE GHRH; CANCER CELLS; EXPRESSION; METASTASIS; APOPTOSIS;

D O I：

10.1016/j.canlet.2010.07.019

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases MMP-2 and MMP 9 decreased E cadherin-mediated cell-cell adhesion and increased cell motility VIP increased cyclin D1 expression and cell proliferation that was blocked after VPAC(1)-receptor siRNA transfection Similar effects were seen in RWPE-1 tumors developed by subcutaneous injection of VIP-treated cells in athymic nude mice VIP acts as a cytokine in RWPE 1 cell transformation conceivably through epithelial-mesenchymal transition (EMT) reinforcing VIP role in prostate tumorigenesis (C) 2010 Elsevier Ireland Ltd All rights reserved

引用

页码：11 / 21

页数：11

共 49 条

[1] Altered apoptotic gene expression and acquired apoptotic resistance in cadmium-transformed human prostate epithelial cells [J].

Achanzar, WE ;

Webber, MM ;

Waalkes, MP .

PROSTATE, 2002, 52 (03) :236-244

[2]

Achanzar WE, 2001, CANCER RES, V61, P455

[3] The metastatic cascade in prostate cancer [J].

Arya, Manit ;

Bott, Simon R. ;

Shergill, Iqbal S. ;

Ahmed, Flashim U. ;

Williamson, Magali ;

Patel, Fliten R. .

SURGICAL ONCOLOGY-OXFORD, 2006, 15 (03) :117-128

[4] Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18 [J].

Bello, D ;

Webber, MM ;

Kleinman, HK ;

Wartinger, DD ;

Rhim, JS .

CARCINOGENESIS, 1997, 18 (06) :1215-1223

[5] GHRH antagonists reduce the invasive and metastatic potential of human cancer cell lines in vitro [J].

Bellyei, Szabolcs ;

Schally, Andrew V. ;

Zarandi, Marta ;

Varga, Jozsef L. ;

Vidaurre, Irving ;

Pozsgai, Eva .

CANCER LETTERS, 2010, 293 (01) :31-40

[6] Mechanisms of acquired androgen independence during arsenic-induced malignant transformation of human prostate epithelial cells [J].

Benbrahim-Tallaa, Lamia ;

Webber, Mukta M. ;

Waalkes, Michael P. .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2007, 115 (02) :243-247

[7]

Birchmeier W, 1996, CURR TOP MICROBIOL, V213, P117

[8] Expression of matrix metalloproteinases (MMP-2 and -9) and their inhibitors (TIMP-1 and -2) in prostate cancer tissue [J].

Brehmer, B ;

Biesterfeld, S ;

Jakse, G .

PROSTATE CANCER AND PROSTATIC DISEASES, 2003, 6 (03) :217-222

[9] CYCLIC AMP-STIMULATING EFFECT OF VASOACTIVE INTESTINAL PEPTIDE IN ISOLATED EPITHELIAL-CELLS OF RAT VENTRAL PROSTATE [J].

CARMENA, MJ ;

PRIETO, JC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 763 (04) :414-418

[10] Invasion and metastasis markers in cancers [J].

Chin, D ;

Boyle, GM ;

Kane, AJ ;

Theile, DR ;

Hayward, NK ;

Parson, PG ;

Coman, WB .

BRITISH JOURNAL OF PLASTIC SURGERY, 2005, 58 (04) :466-474

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