UPLC Analysis of Mycophenolic Acid and Its Phenol and Acyl Glucuronide Metabolites in Human Plasma

被引：3

作者：

Guo, Dong ^{[1
]}

Xu, Lin-Yong ^{[2
]}

Pang, Liang-Fang ^{[1
]}

Tan, Zhi-Rong ^{[1
]}

Han, Yang ^{[1
]}

Yang, Hong ^{[1
]}

Zhou, Gan ^{[1
]}

Chen, Yao ^{[1
]}

Ouyang, Dong-Sheng ^{[1
]}

Zhou, Hong-Hao ^{[1
]}

机构：

[1] Cent S Univ, Inst Clin Pharmacol, Pharmacogenet Res Inst, Changsha 410078, Hunan, Peoples R China

[2] Cent S Univ, Coll Publ Hlth, Changsha 410078, Hunan, Peoples R China

来源：

CHROMATOGRAPHIA | 2010年 / 72卷 / 7-8期

关键词：

Column liquid chromatography; Glucuronide metabolites; Human plasma; Liquid-liquid extraction; Mycophenolic acid; PERFORMANCE LIQUID-CHROMATOGRAPHY; RENAL-TRANSPLANT RECIPIENTS; TANDEM MASS-SPECTROMETRY; ENZYME-MULTIPLIED IMMUNOASSAY; ACUTE REJECTION; HUMAN SERUM; MOFETIL; PHARMACOKINETICS; QUANTIFICATION; IDENTIFICATION;

D O I：

10.1365/s10337-010-1715-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, and reliable UPLC method has been validated for simultaneous analysis of mycophenolic acid (MPA) and its phenol glucuronide (MPAG) and acyl glucuronide (AcMPAG) metabolites in human plasma. Separation was performed on a UPLC BEH C(18) column. The mobile phase was a gradient prepared from 10 mM ammonium formate and acetonitrile; the flow rate was 0.5 mL min(-1), the total run time 5.5 min, and the analytes were quantified at 254 nm. Sample preparation was by liquid-liquid extraction. The method is specific, sensitive, and linear in the concentration ranges 0.062-126 mu g mL(-1) for MPA, 0.080-188 mu g mL(-1) for MPAG, and 0.031-15.9 mu g mL(-1) for AcMPAG.

引用

页码：747 / 752

页数：6

共 33 条

[1] Mycophenolate mofetil and its mechanisms of action [J].

Allison, AC ;

Eugui, EM .

IMMUNOPHARMACOLOGY, 2000, 47 (2-3) :85-118

[2] Sensitive high-performance liquid chromatography-tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine [J].

Benoit-Biancamano, Marie-Odile ;

Carona, Patrick ;

Lévesque, Eric ;

Delage, Robert ;

Couture, Félix ;

Guillemette, Chantal .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 858 (1-2) :159-167

[3] Comparison of a New Enzymatic Assay with a High-Performance Liquid Chromatography/Ultraviolet Detection Method for Therapeutic Drug Monitoring of Mycophenolic Acid in Adult Liver Transplant Recipients [J].

Blanchet, Benoit ;

Taieb, Fabrice ;

Conti, Filomena ;

Abbas, Halim ;

Seydi, Idrissa ;

Harcouet, Laura ;

Dauphin, Alain ;

Calmus, Yvon ;

Tod, Michel .

LIVER TRANSPLANTATION, 2008, 14 (12) :1745-1751

[4]

Brandhorst G, 2008, THER DRUG MONIT, V30, P428, DOI 10.1097/FTD.0b013e31817fd590

[5] Quantification by liquid chromatography tandem mass spectrometry of mycophenolic acid and its phenol and acyl glucuronide metabolites [J].

Brandhorst, Gunnar ;

Streit, Frank ;

Goetze, Sandra ;

Oellerich, Michael ;

Armstrong, Victor William .

CLINICAL CHEMISTRY, 2006, 52 (10) :1962-1964

[6] Pharmacokinetics and bioavailability of mycophenolate mofetil in healthy subjects after single-dose oral and intravenous administration [J].

Bullingham, R ;

Monroe, S ;

Nicholls, A ;

Hale, M .

JOURNAL OF CLINICAL PHARMACOLOGY, 1996, 36 (04) :315-324

[7] Clinical pharmacokinetics of mycophenolate mofetil [J].

Bullingham, RES ;

Nicholls, AJ ;

Kanmm, BR .

CLINICAL PHARMACOKINETICS, 1998, 34 (06) :429-455

[8] The pharmacokinetic-pharmacodynamic relationship for mycophenolate mofetil in renal transplantation [J].

Hale, MD ;

Nicholls, AJ ;

Bullingham, RES ;

Hené, R ;

Hoitsma, A ;

Squifflet, JP ;

Weimar, W ;

Vanrenterghem, Y ;

Van de Woude, FJ ;

Verpooten, GA .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 1998, 64 (06) :672-683

[9] Analytic validation of the enzyme multiplied immunoassay technique for the determination of mycophenolic acid in plasma from renal transplant recipients compared with a high-performance liquid chromatographic assay [J].

Hosotsubo, H ;

Takahara, S ;

Imamura, R ;

Kyakuno, M ;

Tanaka, T ;

Yazawa, K ;

Hanafusa, T ;

Matsumiya, K ;

Nonomura, N ;

Okuyama, A ;

Sugimoto, H .

THERAPEUTIC DRUG MONITORING, 2001, 23 (06) :669-674

[10] Simultaneous determination of mycophenolic acid and its phenolic glucuronide in human plasma using an isocratic high-performance liquid chromatography procedure [J].

Indjova, D ;

Kassabova, L ;

Svinarov, D .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 817 (02) :327-330

← 1 2 3 4 →