A chromosome-level genome assembly of the potato grouper (Epinephelus tukula)

被引：9
作者：
Wang, Linna [1 ,2 ]
Li, Zhentong [1 ,2 ]
Liu, Yang [1 ,2 ]
Chen, Shuai [1 ,3 ]
Li, Linlin [1 ,4 ]
Duan, Pengfei [1 ,3 ]
Wang, Xinyi [1 ,3 ]
Li, Wensheng [5 ]
Wang, Qingbin [5 ]
Zhai, Jieming [5 ]
Tian, Yongsheng [1 ,2 ,5 ]
机构：
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Peoples R China
[3] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
[4] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
[5] Mingbo Aquat Co Ltd, Laizhou 261400, Peoples R China
来源：
GENOMICS | 2022年 / 114卷 / 05期
关键词：
AMP; Epinephelus tukula; Hi-C; Genome assembly; PacBio long-read sequencing; DE-NOVO IDENTIFICATION; GENE; TOOL; ANNOTATION; FAMILIES; SYSTEM; GROWTH; FINDER;
D O I：
10.1016/j.ygeno.2022.110473
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The potato grouper, Epinephelus tukula, is one of the largest coral reef teleost, and it is an important germplasm resource for selection and cross breeding. Here we report a potato grouper genome assembly generated using PacBio long-read sequencing, Illumina sequencing and high-throughput chromatin conformation capture (Hi-C) technology. The genome size was 1.13 Gb, with a total of 508 contigs anchored into 24 chromosomes. The scaffold N50 was 42.65 Mb. For the genome models, our assembled genome contained 98.11% complete BUSCO with the vertebrata_odb9 database. One more copies of Gh and Hsp90b1 were identified in the E. tukula genome, which might contribute to its fast growth and high resistance to stress. In addition, 435 putative antimicrobial peptide (AMP) genes were identified in the potato grouper. This study provides a good reference for whole genome selective breeding of the potato grouper and for future development of novel marine drugs.
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页数：8
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