Cardiogenic effects of characterized Geum urbanum extracts on adipose-derived human mesenchymal stem cells

被引:8
作者
Neshati, Vajiheh [1 ]
Mollazadeh, Samaneh [1 ]
Bazzaz, Bibi Sedigheh Fazly [1 ,2 ]
Iranshahi, Mehrdad [1 ,2 ]
Mojarrad, Majid [3 ,4 ]
Naderi-Meshkin, Hojjat [5 ]
Kerachian, Mohammad Amin [3 ,4 ]
机构
[1] Mashhad Univ Med Sci, Biotechnol Res Ctr, Inst Pharmaceut Technol, Mashhad, Iran
[2] Mashhad Univ Med Sci, Sch Pharm, Mashhad, Iran
[3] Mashhad Univ Med Sci, Med Genet Res Ctr, Mashhad, Iran
[4] Mashhad Univ Med Sci, Fac Med, Dept Med Genet, Mashhad, Iran
[5] ACECR, Stem Cell & Regenerat Med Res Grp, Khorasan Razavi Branch, Mashhad, Iran
关键词
adipose-derived mesenchymal stem cells; cardiogenesis; Geum urbunum; ELLAGIC ACID; IN-VITRO; CARDIOMYOCYTE DIFFERENTIATION; INFARCTED HEART; TISSUE; REPAIR; JAPONICUM; RATS; VIVO;
D O I
10.1139/bcb-2017-0313
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stem cell therapy is considered as a promising treatment for cardiovascular diseases. Adipose-derived mesenchymal stem cells (ADMSCs) have the ability to undergo cardiomyogenesis. Medicinal plants are effective and safe candidates for cell differentiation. Therefore, the aim of our study was to investigate cardiogenic effects of characterized (HPLC-UV) extracts of Geuni urbanum on ADMSCs of adipose tissue. The methanolic extracts of the root and aerial parts of G. urbanum were obtained and MTT assay was used for studying their cytotoxic effects. Then, cells were treated with 50 or 100 mu g/mL of the extracts from root and aerial parts of G. urbanum. MTT assay showed that the extracts of G. urbanum did not have any toxic effects on ADMSCs. Immunostaining results showed increase in the expression of alpha-actinin and cardiac troponin I (cTnI), and quantitative real-time reverse-transcription PCR data confirmed the upregulation of ACTN, ACTC1, and TNNI3 genes in ADMSCs after treatment. According to HPLC fingerprinting, some cardiogenic effects of G. urbanum extracts are probably due to ellagic and gallic acid derivatives. Our findings indicated that G. urbanum extracts effectively upregulated some essential cardiogenic markers, which confirmed the therapeutic role of this plant as a traditional cardiac medicine.
引用
收藏
页码:610 / 618
页数:9
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