Lipase catalyzed production of Monoacylglycerols by the esterification of fish oil fatty acids with glycerol

被引：23

作者：

Byun, Hee-Guk

Eom, Tae-Kil

Jung, Won-Kyo

Kim, Se-Kwon ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Kangnung 608737, South Korea

[2] Kangnung Natl Univ, Fac Sci & Biotechnol, Kangnung 210702, South Korea

[3] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2007年 / 12卷 / 05期

关键词：

monoacylglycerol (MAG); esterification; fish oil; lipase;

D O I：

10.1007/BF02931345

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, we attempted the efficient production of monoacylglycerols (MAG) via the lipase-catalyzed esterification of glycerol with fatty acids obtained from sardine oil. The reaction factors that influenced MAG synthesis were the glycerol to fatty acid mole ratio, amount of enzyme, organic solvent, temperature, and the type of lipase used. Porcine pancreas lipase was selected to catalyze this reaction. The optimum conditions we determined for MAG synthesis were a glycerol to fatty acid mole ratio of 1:6, 100 mg/mL of lipase, and 30 degrees C in dioxane. Under these conditions, the MAG content was 689% (w/w) after 72 h of reaction. The MAGs synthesized via the lipase-catalyzed esterification of glycerol with fatty acids included monomyristin, monopamiltin, and monoolein, as identified by GC-MS. (C) KSBB

引用

页码：491 / 496

页数：6