Factors affecting sporulation of Pestalotiopsis disseminata causing grey blight disease of Persea bombycina Kost., the primary food plant of muga silkworm

The effect of inoculum concentrations, temperature, relative humidity (RH), incubation period and leaf age on sporulation of Pestalotiopsis disseminata and the development of grey blight disease on som (Persea bombycina Kost.) were studied in controlled environmental conditions. These factors had a significant role on spore germination and germ tube development of P. disseminata, the causal organism of the disease. The optimum inoculum concentration of the pathogen were found to be 1 x 10(7) spores ml(-1) for maximum infection percentage. A nonlinear relationship was found between temperature and germination percentage of spores at a given relative humidity (RH). However, 25 degrees C (+/- 2) temperature and 70% RH were the optimum parameters for better spore germination of the pathogen. Young leaves (leaf no. 1-4 from the top) were more susceptible for development of the diseases in a controlled environment than the older leaves. The germination of spores started at 8 h of incubation and gradually increased up to 20 h (maximum). The disease severity was favoured in the optimum range of temperature (25 +/- 2 degrees C) and RH (70%) which had a combined effect. These epidemiological parameters could help in the management practices of the disease. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.