Development and characterization of the first 16 microsatellites loci for Panulirus pascuensis (Decapoda: Palinuridae) from Easter Island using Next Generation Sequencing

被引：5

作者：

Diaz-Cabrera, Ernesto ^{[1
,2
]}

Meerhoff, Erika ^{[2
,3
]}

Rojas-Hernandez, Noemi ^{[1
,2
]}

Vega-Retter, Caren ^{[1
,2
]}

Veliz, David ^{[1
,2
]}

机构：

[1] Univ Chile, Fac Ciencias, Dept Ciencias Ecol, Las Palmeras 3425, Santiago, Chile

[2] Nucleo Milenio Ecol & Manejo Sustentable Islas Oc, Larrondo 1281, Coquimbo, Chile

[3] CEAZA, Ave Ossandon 877, Coquimbo, Chile

来源：

REVISTA DE BIOLOGIA MARINA Y OCEANOGRAFIA | 2017年 / 52卷 / 02期

关键词：

Panulirus pascuensis; Easter Island; microsatellite; genetic marker; Y GOMEZ; CONNECTIVITY; CONSERVATION; PROGRESS; PACIFIC; REEFS;

D O I：

10.4067/S0718-19572017000200018

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

The spiny lobster Panulirus pascuensis stands out among the endemic species of Easter Island, due to its cultural and economic importance. A total of 16 microsatellite loci were characterized in 18 individuals, 9 of which were polymorphic. The mean number of alleles per locus was 3.44 (2-6) and the observed heterozygosity ranged from 0.11 to 0.93. None of the loci exhibited significant linkage disequilibrium or departures from HWE. These new microsatellites will be used to obtain information about migration, population structure and genetic diversity of P. pascuensis in order to improve the future sustainable management and conservation plans.

引用

页码：395 / 398

页数：4

共 30 条

[1]

ABBOTT IA, 1985, P 5 INT COR REEF C T, V5, P71

[2] Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques [J].

Aljanabi, SM ;

Martinez, I .

NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4692-4693

[3] Connectivity, biodiversity conservation and the design of marine reserve networks for coral reefs [J].

Almany, G. R. ;

Connolly, S. R. ;

Heath, D. D. ;

Hogan, J. D. ;

Jones, G. P. ;

McCook, L. J. ;

Mills, M. ;

Pressey, R. L. ;

Williamson, D. H. .

CORAL REEFS, 2009, 28 (02) :339-351

[4]

ANDERSON A, 1995, J POLYNESIAN SOC, V104, P110

[5]

Belkhir K, 1996, 5171 CNRS UMR, V5171

[6] Connectivity between island Marine Protected Areas and the mainland [J].

Bell, James J. .

BIOLOGICAL CONSERVATION, 2008, 141 (11) :2807-2820

[7]

Boyko CB, 2003, EASTER ISLAND: SCIENTIFIC EXPLORATION INTO THE WORLD'S ENVIRONMENTAL PROBLEMS IN MICROCOSM, P155

[8] A review and analysis of Easter Island's traditional and artisan fisheries [J].

Carlos Castilla, Juan ;

Yanez, Eleuterio ;

Silva, Claudio ;

Fernandez, Miriam .

LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH, 2014, 42 (04) :690-702

[9]

CORFO, 1978, PART CORP FOM PROD D

[10] Spatial patterns of endemism in shallow-water reef fish populations of the Northwestern Hawaiian Islands [J].

DeMartini, EE ;

Friedlander, AM .

MARINE ECOLOGY PROGRESS SERIES, 2004, 271 :281-296

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