Nitrogen is considered to be one of the major deficient nutrient elements ill desert soils, where levels of nitrogen and its fractions undergo rapid tempospatial and chemical changes with the changes in season, according to water availability A better understanding of this knowledge would be useful for describing the nitrogen state and changes in arid to semiarid systems. The present study, investigated mineral nitrogen content in soils sampled from four sites in the Judean Desert along a topoclimatic gradient ranging from Mediterranean to arid conditions, over a period of two years. It was concluded that, along the Judean Desert topoclimatic gradient, temporal dynamics in total soluble nitrogen (TSN) (0.4-80.0 mg kg(-1) dry, soil), mainly consisting of NH4+-N (0.03-28.1 mg kg(-1) dry soil) and NO3--N (0-57.2 mg kg(-1) dry soil), are closely related to the soil moisture pattern. However, the effect of soil moisture oil long-term TSN spatial dynamics is not so pronounced, due to leaching and denitrification. Significantly higher NH4+-N than NO3--N levels exist in soils during dry periods, whereas an opposite pattern was observed during the wet seasons. Ammonia may he volatilized, preventing ammonium fi-om accumulating with a decrease in soil clay content. Afore NO2--N accumulated in a drier site than in moister sites, probably due to aridity having a different impact oil different nitrification processes.