Cell block samples from malignant pleural effusion might be valid alternative samples for anaplastic lymphoma kinase detection in patients with advanced non-small-cell lung cancer

被引：40

作者：

Zhou, Jianya ^{[1
]}

Yao, Hongtian ^{[2
]}

Zhao, Jing ^{[2
]}

Zhang, Shumeng ^{[1
]}

You, Qihan ^{[2
]}

Sun, Ke ^{[2
]}

Zou, Yinying ^{[2
]}

Zhou, Caicun ^{[3
]}

Zhou, Jianying ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Thorac Dis Ctr,Dept Resp Dis, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Pathol, Hangzhou 310003, Zhejiang, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai Pulm Hosp, Inst Canc,Dept Med Oncol, Shanghai 200092, Peoples R China

来源：

HISTOPATHOLOGY | 2015年 / 66卷 / 07期

基金：

中国国家自然科学基金;

关键词：

anaplastic lymphoma kinase; fluorescence in-situ hybridization; immunohistochemistry; lung cancer; pleural effusion; reverse transcription polymerase chain reaction; ALK REARRANGEMENT; CYTOLOGICAL SPECIMENS; PROGNOSTIC IMPACT; EGFR MUTATION; ADENOCARCINOMA; CRIZOTINIB; IMMUNOHISTOCHEMISTRY; IDENTIFICATION; ACCURACY; SUCCESS;

D O I：

10.1111/his.12560

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

AimTo evaluate the clinical value of cell block samples from malignant pleural effusion (MPE) as alternative samples to tumour tissue for anaplastic lymphoma kinase (ALK) detection in patients with advanced non-small-cell lung cancer (NSCLC). Methods and resultsFifty-two matched samples were eligible for analysis. ALK status was detected by Ventana immunohistochemistry (IHC) (with the D5F3 clone), reverse transcription polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH) in MPE cell block samples, and by FISH in tumour tissue block samples. In total, ALKFISH results were obtained for 52 tumour tissue samples and 41 MPE cell block samples. Eight cases (15.4%) were ALK-positive in tumour tissue samples by FISH, and among matched MPE cell block samples, five were ALK-positive by FISH, seven were ALK-positive by RT-PCR, and eight were ALK-positive by Ventana IHC. The ALK status concordance rates between tumour tissue and MPE cell block samples were 78.9% by FISH, 98.1% by RT-PCR, and 100% by Ventana IHC. In MPE cell block samples, the sensitivity and specificity of Ventana IHC (100% and 100%) and RT-PCR (87.5% and 100%) were higher than those of FISH (62.5% and 100%). ConclusionsMalignant pleural effusion cell block samples had a diagnostic performance for ALK detection in advanced NSCLC that was comparable to that of tumour tissue samples. MPE cell block samples might be valid alternative samples for ALK detection when tissue is not available. Ventana IHC could be the most suitable method for ALK detection in MPE cell block samples.

引用

页码：949 / 954

页数：6

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