In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM

被引：3
作者：
Greiwe, Julia F. [1 ]
Zanetti, Giulia [2 ]
Miller, Thomas C. R. [1 ,3 ]
Costa, Alessandro [1 ]
机构：
[1] Francis Crick Inst, Macromol Machines Lab, London NW1 1AT, England
[2] Birkbeck Coll, Inst Struct & Mol Biol, Malet St, London WC1E 7HX, England
[3] Univ Copenhagen, Ctr Chromosome Stabil, Dept Cellular & Mol Med, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2022年 / 72卷
基金：
英国生物技术与生命科学研究理事会; 欧洲研究理事会; 欧盟地平线“2020”; 英国惠康基金;
关键词：
DOUBLE-HEXAMER; ORIGIN; COMPLEX; MECHANISMS; VISUALIZATION;
D O I：
10.1016/j.sbi.2021.11.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA replication has been reconstituted in vitro with yeast proteins, and the minimal system requires the coordinated assembly of 16 distinct replication factors, consisting of 42 polypeptides. To understand the molecular interplay between these factors at the single residue level, new structural biology tools are being developed. Inspired by advances in singlemolecule fluorescence imaging and cryo-tomography, novel single-particle cryo-EM experiments have been used to characterise the structural mechanism for the loading of the replicative helicase. Here, we discuss how in silico reconstitution of single-particle cryo-EM data can help describe dynamic systems that are difficult to approach with conventional three-dimensional classification tools.
引用
收藏
页码：279 / 286
页数：8
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