Development of a real-time PCR method for quantification of Prevotella histicola from the gut

被引：8
|
作者：
Balakrishnan, Baskar [1 ]
Luckey, David [1 ]
Marietta, Eric [2 ]
Karau, Melissa [3 ]
Patel, Robin [3 ]
Murray, Joseph [2 ]
Taneja, Veena [1 ]
机构：
[1] Mayo Clin, Dept Immunol, Rochester, MN 55905 USA
[2] Mayo Clin, Dept Gastroenterol, Rochester, MN 55905 USA
[3] Mayo Clin, Dept Clin Microbiol, Rochester, MN 55905 USA
来源：
ANAEROBE | 2017年 / 48卷
关键词：
Prevotella histicola; Real-time PCR quantification qPCR; Anaerobe; Gut microbiome; IDENTIFICATION; POPULATIONS; MICROBIOME; ARTHRITIS; BACTERIA; PRIMERS;
D O I：
10.1016/j.anaerobe.2017.06.019
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 10(3) colony-forming units (CFU) in the gut. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：37 / 41
页数：5
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